FastQCFastQC Report
Wed 25 May 2016
SRR1780092_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780092_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56445
Sequences flagged as poor quality0
Sequence length76
%GC93

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4365677.34254584108425No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG6451.1427052883337763No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1620.2870050491629019No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG750.13287270794578793No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG630.11161307467446187No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590.1045265302506865No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590.1045265302506865No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAC150.002213920770.0000142
CAGTCAC150.002213920770.0000137
TATATCA150.002213920770.000015
TGGTATC150.002213920770.0000164
GCGATCT150.002213920770.0000128
TATGCTA150.002213920770.0000156
TCGGCTA150.002213920770.0000151
GTACTAT150.002213920770.0000145
CAGTACT150.002213920770.0000143
TAACGGG150.002213920770.0000116
GACGGTC150.002213920770.0000122
ATGGTAT150.002213920770.0000163
GATAACG150.002213920770.0000114
GTCGAAA150.002213920770.0000126
GGATATA150.002213920770.000012
AGTACTA150.002213920770.0000144
ATAACGG150.002213920770.0000115
AGCAGTC150.002213920770.0000135
GCTATGC150.002213920770.0000154
TCAAAGA150.002213920770.000019
Produced by FastQC (version 0.11.5)