FastQCFastQC Report
Wed 25 May 2016
SRR1780092_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780092_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56445
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2518444.616883692089644No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG574910.185135973071132No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC16772.9710337496678183No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG11281.9984055275046506No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT11241.9913189830808753TruSeq Adapter, Index 6 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC8541.5129772344760386No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT5651.000974399858269No Hit
CTTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCGT4600.8149526087341661No Hit
CTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC4240.7511737089201878No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACGT3990.7068828062715918No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTAAGCCGT3340.5917264593852423No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGA2400.42519266542652134No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACACCATCTCGTATGCCGT2120.3755868544600939No Hit
TCTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC1980.35078394897688014No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGAATGCCGT1810.3206661351758349No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCAGT1760.31180795464611566No Hit
CCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1650.29231995748073347No Hit
TATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCT1650.29231995748073347No Hit
TTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC1580.2799185047391266No Hit
CTTTACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC1520.26928868810346357No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCAT1520.26928868810346357No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCACGTATGCCGT1510.26751705199751974No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTAGTATGCCGT1490.263973779785632No Hit
CTTATACACATCTCCGAGACCACGAGACACCACAACATCTCGTATGCCGT1370.24271414651430598No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACAACTCGTATGCCGT1370.24271414651430598No Hit
CTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG1260.22322614934892374No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCAG1210.21436796881920453No Hit
CTTATACACATCTCCGAGCCCACGAGACCCCACAACATCTCGTATGCCGT1080.19133669944193463No Hit
CTTATACACATCTCCGAGCCCACGAGACAACACAACATCTCGTATGCCGT1040.18425015501815925No Hit
CTTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG950.16830543006466472No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATATCGTATGCCGT950.16830543006466472No Hit
CTTATACACATCTCCGAGCACACGAGACACCACAACATCTCGTATGCCGT920.1629905217468332No Hit
TCTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT840.14881743289928248No Hit
CTTATACACATCTCCGAGCCAACGAGACACCACAACATCTCGTATGCCGT760.13464434405173178No Hit
CTTATACACATCTCCGAGCCCACGAGACACCAAAACATCTCGTATGCCGT750.13287270794578793RNA PCR Primer, Index 28 (95% over 23bp)
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGACG750.13287270794578793No Hit
TCTTATACACATCTCCGAGCCCACGAGCCACCACAACATCTCGTATGCCG730.12932943573390027No Hit
CTTATACACATATCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT730.12932943573390027No Hit
TCCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC700.12401452741606875No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAAAATCTCGTATGCCGT700.12401452741606875No Hit
CATATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT680.12047125520418106No Hit
TCTTATACACATCTCCGAGCCCACGAGACACAACAACATCTCGTATGCCG650.11515634688634956No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACCTCTCGTATGCCGT640.1133847107804057No Hit
CTTATACACATCTCAGAGCCCACGAGACACCACAACATCTCGTATGCCGT620.10984143856851802No Hit
CAGCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGC620.10984143856851802No Hit
CTTATACACATCTCCGAGCCCACGAGCCACCACAACATCTCGTATGCCGT620.10984143856851802No Hit
GCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG580.10275489414474266No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT570.10098325803879883No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCAC150.002213920770.0000127
ACTTGAA150.002213920770.0000156
CGCCACA150.002213920770.0000128
ACACCAT455.456968E-1270.0000132
TAAGACG150.002213920770.0000143
CCCACAA301.0067197E-770.0000129
CAGAGAC150.002213920770.0000114
AAAAGAA150.002213920770.0000159
GCAGTAA150.002213920770.0000146
CTTATAA455.456968E-1270.000011
GTAAGAC150.002213920770.0000142
TCGTATT150.002213920770.0000140
CCCCACA301.0067197E-770.0000128
AAAGAAA150.002213920770.0000160
GAGACCC353.6288839E-970.0000124
CTCGAAA207.863532E-570.039
ACGTCAT207.863532E-570.047
GACGTCA207.863532E-570.046
TTCTTGA207.863532E-570.055
AGACGCC252.8060986E-670.025
Produced by FastQC (version 0.11.5)