FastQCFastQC Report
Wed 25 May 2016
SRR1780091_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780091_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65787
Sequences flagged as poor quality0
Sequence length76
%GC94

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5337581.13305060270267No Hit
CTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGTG9901.504856582607506No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2240.34049280252937514No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1230.18696702996032652No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1010.1535257725690486No Hit
TCTTATACACATCTGACGCTGCCGACGACGATACACGTGTAGATCTCGGT780.1185644580236217No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG670.10184382932798272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGTGTC150.002215561270.0000140
CGGGTGT150.002215561270.000017
CGGCCGG150.002215561270.0000118
GTGTGGC150.002215561270.0000110
TGTCGGG150.002215561270.0000143
GTGTCGG207.873244E-570.042
TTGTGGG252.8112954E-670.02
GGTGTCG207.873244E-570.041
CATTAAA1300.061.9230861
TATCATT1300.061.9230858
ATCATTA1300.061.9230859
TCATTAA1300.061.9230860
TGTGCGC401.0464646E-861.253
GTATCAT1400.060.00000457
ATTAAAA1400.060.00000462
GTGGTCG1400.060.00000448
GTGCGCG352.932702E-760.0000044
CGTATCA1350.059.62962756
GGTCGCC1350.059.62962750
TGGTCGC1300.059.23077449