FastQCFastQC Report
Wed 25 May 2016
SRR1780091_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780091_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65787
Sequences flagged as poor quality0
Sequence length76
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT3456652.54229559031419No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG700210.64344019335127No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC23123.5143721403924784No Hit
ATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT16812.5552160761244624Illumina PCR Primer Index 10 (95% over 22bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG15492.3545685317767946No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC11171.6979038411844287No Hit
CTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC5830.8861933208688647No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT4410.6703452049797072No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGACGT4070.6186632617386414No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCAGT2820.42865611747001686No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTAATCTCGTATGCCGT2730.4149756030826759No Hit
TATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCT2480.37697417422895096Illumina PCR Primer Index 10 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATATCGTATGCCGT2340.35569337407086504No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC2280.3465730311459711No Hit
TTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC2220.3374526882210771No Hit
CTTTACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTC2200.3344125739127791No Hit
CTTATACACATCTCCGAGACCACGAGACTTAAACTTATCTCGTATGCCGT1990.3024913736756502No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG1820.27665040205511726No Hit
CTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG1790.2720902305926703No Hit
CTTATACACATCTCCGAGCCCACGAGACTAAAACTTATCTCGTATGCCGT1760.2675300591302233No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG1730.2629698876677763No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCAG1670.2538495447428823No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGA1650.25080943043458437No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTAACTCGTATGCCGT1480.22496845881405142No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACATATCTCGTATGCCGT1460.2219283445057534No Hit
CTTATACACATCTCCGAGCCAACGAGACTTAAACTTATCTCGTATGCCGT1250.1900071442686245No Hit
CTTATACACATCTCCGAGCCCACGAGACATAAACTTATCTCGTATGCCGT1250.1900071442686245No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGAATGCCGT1200.18240685849787952No Hit
TCTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT1100.16720628695638956No Hit
CTTATACACATCTCCGAGCACACGAGACTTAAACTTATCTCGTATGCCGT1030.1565658868773466No Hit
CTTATACACATCTCAGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT980.14896560110660162No Hit
TCCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC970.1474455439524526No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAACCTTATCTCGTATGCCGT950.14440542964415462No Hit
CTTATACACATATCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT880.13376502956511166No Hit
CTTATAAACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT830.12616474379436665No Hit
CTTATACACATCTACGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT770.11704440086947268No Hit
GCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG760.1155243437153237No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAAATTATCTCGTATGCCGT760.1155243437153237No Hit
CATATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT700.10640400079042972No Hit
CTTATACAAATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT690.10488394363628072No Hit
TCTTATACACATCTCCGAGCCAACGAGACTTAAACTTATCTCGTATGCCG670.10184382932798272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTATGCT150.002215561270.0000152
ACTAAAA650.070.0000127
AACAAAT150.002215561270.0000132
TAAACAA150.002215561270.0000130
CTTTACA301.009339E-770.000011
TCGAATG301.009339E-770.0000140
TAAAACT550.070.0000129
GAATGCC150.002215561270.0000142
TGCCTTC150.002215561270.0000145
CTTATGC150.002215561270.0000151
CTAAAAC550.070.0000128
TCCGCCA150.002215561270.0000110
AATATCC150.002215561270.000019
AATGCCG150.002215561270.0000143
TTTACAC353.6416168E-970.000012
ATCTCGA207.873244E-570.037
TATACAA401.3278623E-1070.03
TCTCGAA207.873244E-570.038
CGAATGC252.8112954E-670.041
AAAACTT500.070.030
Produced by FastQC (version 0.11.5)