FastQCFastQC Report
Wed 25 May 2016
SRR1780090_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780090_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174886
Sequences flagged as poor quality0
Sequence length76
%GC71

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7503142.90280525599533No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG2595114.838809281474788No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT24151.3808995574259804No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG17961.026954701920108No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC15360.8782864265864619No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG7760.44371762176503554No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3620.20699198334915317No Hit
CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG3090.1766865272234484No Hit
CTTATACACATCTGACGCTGCCGACGACATGGTTAGTGTAGATCTCGGTG2920.16696590922086388No Hit
CTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT2160.12350902873872122No Hit
CTTTACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG2030.11607561497203893No Hit
CTTATACACATCTGACGCTGCCGACGACTAGGTTAGTGTAGATCTCGGTG1890.10807040014638107No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTGGCG455.456968E-1270.0000111
CTTCGTT150.002221742470.028
GAGGATC150.002221742470.061
GTGCACG150.002221742470.04
AGCCGTA150.002221742470.053
GAACTAG150.002221742470.041
ACTTCGT150.002221742470.027
TGACCTA150.002221742470.010
TATCTCG150.002221742470.015
ATTCACC150.002221742470.023
CGACTTT150.002221742470.025
CGACTTC150.002221742470.025
ATCGGAA150.002221742470.027
CGAATCA550.063.6363656
CTGGCGC550.063.6363612
GCCGAAT453.8198777E-1062.22222554
CGACTAG401.060107E-861.2525
TGGCGGC352.9612966E-760.019
GCGCGTC308.350538E-658.3333326
AGACGCA308.350538E-658.33333213
Produced by FastQC (version 0.11.5)