FastQCFastQC Report
Wed 25 May 2016
SRR1780090_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780090_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences174886
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3898122.28937707992635No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT3406919.480690278238395No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT94375.396086593552371No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG54403.110597760827053No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT50652.8961723637112176No Hit
ATTAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT20591.1773383804306805No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC14950.854842583168464No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC6630.3791041021007971No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT5940.3396498290314834RNA PCR Primer, Index 41 (95% over 22bp)
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT5930.3390780279725078No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT4500.2573104765390025No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT3740.2138535960568599No Hit
CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC3630.2075637844081287No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC3290.18812254840295964No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT2920.16696590922086388No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT2860.1635351028670105No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT2580.1475246732156948No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT2480.14180666262593916No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT2390.13666045309515912No Hit
CTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG2380.13608865203618356No Hit
CTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2350.1343732488592569No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT2160.12350902873872122No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGCCATCTCGTATGCCGT2140.1223654266207701No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC2110.12065002344384343No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG1920.10978580332330774No Hit
CTTAAACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT1880.10749859908740553No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT1880.10749859908740553No Hit
ATTAAACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT1750.1000651853207232No Hit
CTCTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC1750.1000651853207232No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGAT150.00221763970.0326223
ACCGATA150.00221763970.0326224
ATATCGC150.00221763970.0326228
CAATACG150.00221763970.0326228
CGCCCGG150.00221763970.0326228
ATCTTAT353.6834535E-970.012591
ATACGAC150.002220157570.0125830
ATCGCCG150.002220157570.0125830
TACATGA207.914021E-569.9925755
CGAATGC401.3460522E-1069.9925741
CGGACAG150.002222678269.9925641
GTGCACT150.002222678269.992569
AAGTACA150.002222678269.9925666
AACAACG150.002222678269.9925610
CCGATAC150.002222678269.9925634
ATCGTAA150.002222678269.9925639
GACCACT150.002222678269.9925643
CGAGGAT150.002222678269.9925614
AGACCGC150.002222678269.9925617
GTCAAAC150.002222678269.9925611
Produced by FastQC (version 0.11.5)