FastQCFastQC Report
Wed 25 May 2016
SRR1780089_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780089_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175197
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5835433.30764796200848No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG1942811.089230979982535No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT18281.0433968618184102No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG13720.7831184323932489No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC12260.6997836720948418No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG5870.33505139928195116No Hit
CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG2500.14269650736028586No Hit
CTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT2050.1170111360354344No Hit
CTTATACACATCTGACGCTGCCGACGACATGGTTAGTGTAGATCTCGGTG1760.10045834118164124No Hit
CTTTACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG1760.10045834118164124No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAGC150.002221820269.99943521
TGACGAG150.002221820269.99943532
AAGCAAC150.002221820269.99943548
GTCGTAT150.002221820269.99943555
TCGTATC150.002221820269.99943556
CGTCGTA150.002221820269.99943554
GCGTGTG207.910216E-569.9994358
CCGCGAG150.002221820269.99943520
CGCGTGT150.002221820269.9994357
CGCGTCT207.910216E-569.9994357
GCTCCGT150.002221820269.99943540
CGACGAA308.3509985E-658.3328622
TGTGCGC509.749783E-1055.9995463
AAAAAGG12200.054.8102870
CGTATCC200.006942474752.49957757
TCCGATC200.006942474752.4995773
GCGCGTG200.006942474752.4995776
CATGACG200.006942474752.49957730
GAACTCT200.006942474752.49957760
CACGCGT200.006942474752.49957750
Produced by FastQC (version 0.11.5)