FastQCFastQC Report
Wed 25 May 2016
SRR1780089_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780089_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175197
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2880416.440920792022695No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT2610814.902081656649372No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT94005.365388676746748No Hit
CTTAAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT44402.534289970718677No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG42822.444105778066976No Hit
ATTAAACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT22331.2745652037420734No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC10240.5844848941477309No Hit
ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT7620.43493895443415126Illumina PCR Primer Index 5 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC5870.33505139928195116No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC3330.19007174780390076No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT2760.15753694412575558No Hit
CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC2440.139271791183639No Hit
CTAATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT2080.11872349412375782No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTGATC150.002220058870.0134229
TCGTTGT150.002220058870.0134234
CTCGTCA150.002220058870.0134229
GATCGGG150.002220058870.0134232
ATAGATC301.0178701E-770.0134233
AAAACGG207.902387E-570.0134267
ATTGAGC150.002220058870.0134233
GCACTCG150.002220058870.0134226
AAGCCGT252.8325212E-669.9934444
CGACTTC252.8325212E-669.9934448
CGTGGCT207.913575E-569.9934314
TCCGAGA353.6925485E-969.9934313
TCGATTA150.00222257569.9934344
ATCCAGA150.00222257569.993431
ATCGGAC150.00222257569.9934359
CAATACG150.00222257569.9934369
CCGCACT150.00222257569.9934351
ATCGATT150.00222257569.9934343
ATTATAC11600.066.674781
CTTAAAC6150.066.5791241
Produced by FastQC (version 0.11.5)