FastQCFastQC Report
Wed 25 May 2016
SRR1780088_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780088_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230864
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6225426.965659435858342No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG185798.047595120937002No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT18780.8134659366553468No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG14140.6124818074710653No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG5550.24040127520964724No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC4210.18235844479866933No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2320.10049206459214083No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGCGG150.00222264770.05
ATCCCAC150.00222264770.052
GGGCGCT401.0621079E-861.2513
GTCGGGC252.3870228E-455.99999610
TGCCGGC509.767973E-1055.99999619
ACGACAT452.691013E-854.44444324
TCGCCGT26850.053.05400552
GCCGTAT26750.052.99065454
GTAGATC27000.052.7592638
ATCTCGG26950.052.72727642
GTCGCCG27150.052.72559751
TCTCGGT26850.052.6629443
GATCTCG27000.052.62962741
AGATCTC26950.052.59740440
GGTGTCG200.006945036452.541
GTCGACG200.006945036452.551
GTCTGAC200.006945036452.510
CGACGTA200.006945036452.553
TATGCGC200.006945036452.53
TGACGAC407.458075E-752.519