FastQCFastQC Report
Wed 25 May 2016
SRR1780088_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780088_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences230864
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2912612.616085660821955No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2670011.565250537112759No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT102254.429014484718276No Hit
CTTAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT49192.1306916626238825No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG48892.117696999099037No Hit
ATTAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT24611.0659955644881836No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC12520.5423106244368979No Hit
ATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT8870.38420888488460736TruSeq Adapter, Index 8 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC6250.2707221567676208No Hit
ATCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC3380.1464065423799293No Hit
CTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC3230.1399092106175064No Hit
CTAATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2940.1273477025434888No Hit
CTAAAACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2390.10352415274793819No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCAAGC150.00222144570.0095462
GCCGTAT150.00222144570.0095446
AATATCG550.070.0095336
GTATTCT150.002225266569.9792149
ACTCCGA800.065.6055111
CGTATGA1450.065.1812941
TGACGTC1350.064.8236445
ATTATAC12000.064.758821
AGGGGGG55550.064.4012170
ATCGTAT600.064.1754139
ATATCGT600.064.1754137
TTAAACA10550.064.0371552
AAACACA12550.061.641864
TAAACAC11100.061.494863
CGAATGC401.0610165E-861.25834341
AACACAT12250.060.8654335
GTATGAC1500.060.6749342
CGTCTAC352.9715738E-759.9821848
AAGCCGT308.352228E-658.34127844
GTCTTCA900.058.31600649
Produced by FastQC (version 0.11.5)