FastQCFastQC Report
Wed 25 May 2016
SRR1780087_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780087_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117408
Sequences flagged as poor quality0
Sequence length76
%GC82

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7326362.40034750613246No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG2099217.879531207413464No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT18971.6157331698010358No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG11390.9701212864540747No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC10810.9207209048787135No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG5800.49400381575361135No Hit
CTTATACACATCTGACGCTGCCGACGACATGGTTAGTGTAGATCTCGGTG2420.20611883346961027No Hit
CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG2260.1924911420005451No Hit
CTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT2100.17886345053147998No Hit
CTTATACACATCTGACGCTGCCGACGACTAGGTTAGTGTAGATCTCGGTG1800.15331152902698283No Hit
CTTTACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG1520.12946306895611884No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGATAGTGTAGATCTCGGTG1380.11753883892068684No Hit
TATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGT1260.10731807031888797No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTTGGA252.8251252E-670.0000127
CTTGGAT252.8251252E-670.0000128
CTAATAC252.8251252E-670.000011
TCAGAAG252.8251252E-670.0000111
CAGAAGC252.8251252E-670.0000112
TTGGATA252.8251252E-670.0000129
ATGGGCG150.002219918470.04
ACGCTGA207.899049E-570.015
TAAACAT150.002219918470.05
CTTTACA301.0162876E-770.01
AAACATC150.002219918470.06
AGAAGGG150.002219918470.069
AATCATT301.0162876E-770.058
TAAGTGT207.899049E-570.033
TAGCCGT207.899049E-570.052
CTGACGA207.899049E-570.018
CGTGGGC150.002219918470.01
CCGTATA150.002219918470.055
GAGTAGA150.002219918470.036
AACTAGG207.899049E-570.042
Produced by FastQC (version 0.11.5)