FastQCFastQC Report
Wed 25 May 2016
SRR1780087_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780087_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117408
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3193127.19661351866994No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2799123.840794494412645No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1578913.447976287816843No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG55944.764581629871899No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT40493.448657672390297No Hit
ATTAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT29722.5313436903788498No Hit
ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT8940.7614472608340147TruSeq Adapter, Index 18 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC7140.608135731807032No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC7030.5987666939220495No Hit
CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT4970.4233101662578359No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC3960.33728536385936225No Hit
CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC3270.27851594439901883No Hit
ATTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT2860.2435949850095394No Hit
CTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2720.23167075497410738No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC2370.2018601798855274No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT2210.18823248841646226No Hit
CTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT2170.18482556554919596No Hit
TCTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCG2040.17375306623058054No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCG1970.16779095121286455No Hit
CTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1960.16693922049604795No Hit
TCTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1920.1635322976287817No Hit
ATAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1860.15842191332788227No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1780.1516080675933497No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT1730.14734941400926682No Hit
TTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC1720.14649768329245025No Hit
CTTTACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC1710.1456459525756337No Hit
CTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1570.13372172254020168No Hit
ATACCCATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT1500.1277596075224857TruSeq Adapter, Index 18 (95% over 21bp)
ATAAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1470.12520441537203597No Hit
CTAAAACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1470.12520441537203597No Hit
ATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC1460.1243526846552194No Hit
CTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1360.11583537748705369No Hit
TATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCT1290.10987326246933769No Hit
ATTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1250.1064663396020714No Hit
ATTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT1220.10391114745162171No Hit
CCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG1200.10220768601798855No Hit
CTTATACACATCTCCGAGCCCACGAGACCAGGGCGCATCTCGTATGCCGT1190.10135595530117197No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCC455.456968E-1270.0498727
CCGTGCG150.002213652270.0498729
GCCCACA455.456968E-1270.0498731
ACCCGCG150.002213652270.0498727
CGGGCTC150.002213652270.0498730
CCCGCGC150.002213652270.0498728
TCGCCCA455.456968E-1270.0498729
CCGGGCT150.002213652270.0498729
CCGGGCC150.002213652270.0498729
AGCGCAT150.002213652270.0498732
CTCGCCC455.456968E-1270.0498728
GGGCTCA252.8131853E-670.0498731
GACCCGC207.8712095E-570.04986626
CCGCGCG207.8712095E-570.04986629
CCAGACC207.887854E-570.0200323
CCACCAG207.887854E-570.0200320
CAGACCC207.887854E-570.0200324
CCGCGAC150.002221150669.9902114
CTATATA150.002221150669.990212
TATATAC150.002221150669.990213
Produced by FastQC (version 0.11.5)