FastQCFastQC Report
Wed 25 May 2016
SRR1780086_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780086_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177215
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4871527.489208024151456No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG1861410.503625539598792No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT16530.9327652851056627No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG13560.7651722483988376No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC12010.6777078689727167No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG5860.3306717828626245No Hit
CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG2360.13317157125525492No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTCGAC150.002222040269.99802450
GGATCTT150.002222040269.99802418
TAGCCCG150.002222040269.99802410
GGCGCGT207.911259E-569.9980245
AATAGAC150.002222040269.9980247
GCTTTAG150.002222040269.9980241
AGCCGAC452.6868292E-854.4429119
CGTATCA27950.054.09507456
GCCGTAT27850.053.78663654
CGCCGTA28000.053.7484953
GTATCAT27750.053.72821457
TCGCCGT27950.053.71941452
TGGTCGC28150.053.3377549
CGGTGGT28300.053.30238346
GATCTCG28450.053.2673941
CCGTATC28200.053.2431855
GTAGATC28600.053.2327638
GGTGGTC28600.053.2327647
GTCGCCG28300.053.1787151
CTCGGTG28300.053.1787144