FastQCFastQC Report
Wed 25 May 2016
SRR1780086_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780086_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences177215
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2793415.762774031543605No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT2081811.747312586406343No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT71884.056090060096492No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG31631.784837626611743No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT29131.6437660468921933No Hit
ATTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT13570.7657365347177157No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC12990.7330079282227803No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT6390.360578957763169No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC4700.26521456987275344No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT3780.21330022853595915No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT3330.1879073441864402RNA PCR Primer, Index 26 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC3080.17380018621448523No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT2330.13147871229862032No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT2330.13147871229862032No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT2060.11624298168890895No Hit
CTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC1960.11060011850012696No Hit
CTTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT1780.10044296476031937No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTGC252.8311497E-669.9997251
GTCGTCT252.8311497E-669.9997249
CGTCGTC252.8311497E-669.9997248
TAACTCG150.00222182769.9997236
CTTAAAC4350.065.995371
AGGGGGG38150.065.41257570
ATTATAC10850.065.179421
ACTCCGA700.064.9997411
CGAATGC501.4551915E-1162.9997541
GACGTAC453.8198777E-1062.22197326
TTAAACA7300.061.8490682
TAAACAC7350.061.428323
CGTACGA401.0602889E-861.24975228
ACGAGTA401.0602889E-861.24975231
GTACGAG401.0602889E-861.24975229
AACACAT8250.061.090665
TCTTGAA352.9616058E-759.99975656
CGTCTAC352.9616058E-759.99975648
GAGACGA1000.059.4997624
AAACACA9150.058.5243574
Produced by FastQC (version 0.11.5)