##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780086_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 177215 Sequences flagged as poor quality 0 Sequence length 76 %GC 55 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.619704878255227 32.0 27.0 32.0 22.0 32.0 2 30.314815337302147 32.0 32.0 32.0 27.0 32.0 3 30.391326919278843 32.0 32.0 32.0 27.0 32.0 4 30.24970233896679 32.0 32.0 32.0 27.0 32.0 5 26.246722907203115 32.0 22.0 32.0 13.0 32.0 6 33.4756030810033 37.0 32.0 37.0 22.0 37.0 7 34.49446152978021 37.0 37.0 37.0 27.0 37.0 8 33.817030161103744 37.0 37.0 37.0 27.0 37.0 9 35.1940129221567 37.0 37.0 37.0 32.0 37.0 10-11 34.6756510453404 37.0 37.0 37.0 29.5 37.0 12-13 35.07089975453545 37.0 37.0 37.0 32.0 37.0 14-15 35.08099483678018 37.0 37.0 37.0 32.0 37.0 16-17 34.34496233388822 37.0 37.0 37.0 29.5 37.0 18-19 34.776457974776406 37.0 37.0 37.0 27.0 37.0 20-21 34.97845554834523 37.0 37.0 37.0 32.0 37.0 22-23 34.494111672262505 37.0 37.0 37.0 29.5 37.0 24-25 34.856064667212145 37.0 37.0 37.0 32.0 37.0 26-27 34.94535169144824 37.0 37.0 37.0 29.5 37.0 28-29 35.01043929689925 37.0 37.0 37.0 32.0 37.0 30-31 34.89549699517535 37.0 37.0 37.0 32.0 37.0 32-33 34.942987331772144 37.0 37.0 37.0 32.0 37.0 34-35 34.950139660863925 37.0 37.0 37.0 32.0 37.0 36-37 34.764111390119346 37.0 37.0 37.0 29.5 37.0 38-39 34.21442880117372 37.0 37.0 37.0 27.0 37.0 40-41 31.591239454899416 37.0 29.5 37.0 13.0 37.0 42-43 32.1574951330305 37.0 32.0 37.0 13.0 37.0 44-45 32.73829529103067 37.0 32.0 37.0 13.0 37.0 46-47 31.702443359760743 37.0 29.5 37.0 13.0 37.0 48-49 31.816527946279944 37.0 29.5 37.0 13.0 37.0 50-51 30.77724233275964 37.0 29.5 37.0 13.0 37.0 52-53 30.457119882628447 37.0 27.0 37.0 10.5 37.0 54-55 29.495722709702903 37.0 22.0 37.0 10.5 37.0 56-57 30.63075360437886 37.0 27.0 37.0 13.0 37.0 58-59 30.582693338600006 37.0 27.0 37.0 10.5 37.0 60-61 31.425993849279124 37.0 29.5 37.0 13.0 37.0 62-63 33.13047992551421 37.0 32.0 37.0 17.5 37.0 64-65 33.56915893124172 37.0 34.5 37.0 22.0 37.0 66-67 33.57509522331631 37.0 32.0 37.0 22.0 37.0 68-69 33.09361510030189 37.0 32.0 37.0 22.0 37.0 70-71 31.283353553593095 37.0 29.5 37.0 13.0 37.0 72-73 32.83927997065712 37.0 32.0 37.0 17.5 37.0 74-75 33.75218519876985 37.0 37.0 37.0 22.0 37.0 76 33.69864853426629 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.49238081643385456 11101 2 0.18744153061970792 11101 3 0.6380413058865635 11101 4 -0.22373752826586824 11101 5 0.08439105658938928 11101 6 -0.562725118622744 11101 7 0.13144339713063147 11101 8 0.5642760760100316 11101 9 -0.22287478126053628 11101 10-11 -0.2657351972901054 11101 12-13 -0.9559465749738862 11101 14-15 -0.27739081582557645 11101 16-17 -0.11744202196958042 11101 18-19 -0.08257609638182828 11101 20-21 -0.5219181403628284 11101 22-23 0.6847940063570093 11101 24-25 -0.23848280846322467 11101 26-27 -0.4227991614573199 11101 28-29 0.30655398852291427 11101 30-31 -0.30329107050330606 11101 32-33 -0.4059349581991185 11101 34-35 -0.6198930510805525 11101 36-37 -0.14556235675934204 11101 38-39 -0.919240244348245 11101 40-41 -0.8291756255734235 11101 42-43 -0.6331021033531137 11101 44-45 0.05680844501713267 11101 46-47 -0.4281881298810717 11101 48-49 -0.07332895175808929 11101 50-51 0.03436585213674448 11101 52-53 0.34162162730999057 11101 54-55 0.5219486157005342 11101 56-57 -0.4592065390291147 11101 58-59 -0.41194421086191824 11101 60-61 -0.0021895241041285374 11101 62-63 0.11847344225009948 11101 64-65 0.36993997816991 11101 66-67 0.6451709403740225 11101 68-69 0.23041842140393953 11101 70-71 -1.1256733842368476 11101 72-73 0.30768561233198 11101 74-75 -0.44666448292440464 11101 76 -0.24006949487462492 11102 1 -0.25944771180263615 11102 2 0.2804089405682504 11102 3 -0.4067271332214979 11102 4 -0.597493960512864 11102 5 -2.079416833633598 11102 6 -0.618814312447789 11102 7 -0.3069785582724549 11102 8 -0.652705056065443 11102 9 0.5165763336622788 11102 10-11 -1.2785996912866722 11102 12-13 -0.17790197806136376 11102 14-15 -0.39805976951338806 11102 16-17 -0.3339085742851893 11102 18-19 -0.5288025114761652 11102 20-21 -0.761540781872263 11102 22-23 -0.87875659398604 11102 24-25 -0.6262186575198285 11102 26-27 -0.799728835556806 11102 28-29 -0.0021938673947445864 11102 30-31 -0.28528077890811687 11102 32-33 -0.6465009959349644 11102 34-35 -0.33824639584899074 11102 36-37 -0.6103994065020544 11102 38-39 -0.9845918738508175 11102 40-41 -0.7464140475288232 11102 42-43 -0.434302789459462 11102 44-45 -0.7250097368010486 11102 46-47 -1.3938828125568818 11102 48-49 -1.3391093977272135 11102 50-51 -1.9242962404876138 11102 52-53 -0.8193560742337489 11102 54-55 -0.6737632196339405 11102 56-57 -1.1601499352555287 11102 58-59 -0.6885308317881638 11102 60-61 -0.8370094211881742 11102 62-63 -0.6247512575783674 11102 64-65 -1.1677958708866925 11102 66-67 -1.777813622233186 11102 68-69 -1.7872488170180176 11102 70-71 -0.6273028868097477 11102 72-73 -1.2518341132254847 11102 74-75 -1.2286541913292126 11102 76 -0.6918705240341438 11103 1 -0.4850255238211254 11103 2 0.549130049967328 11103 3 0.8518244846521519 11103 4 -1.4045817879396765 11103 5 -0.16185134827150094 11103 6 -0.05009320304871068 11103 7 0.2019582676135272 11103 8 0.20322714732439096 11103 9 -1.1125453920696202 11103 10-11 -0.8059494601618695 11103 12-13 0.3724832299509586 11103 14-15 -0.4241768727491362 11103 16-17 -0.05756034624204176 11103 18-19 -0.8940567487643065 11103 20-21 -0.8064560361095516 11103 22-23 -0.7090339437549176 11103 24-25 -0.7931678100621937 11103 26-27 -0.25658553817621765 11103 28-29 -0.24333408311123605 11103 30-31 -0.5029233058726703 11103 32-33 -0.25243319932479125 11103 34-35 -0.5017286762804218 11103 36-37 -0.5599371568872584 11103 38-39 -0.07657954719442728 11103 40-41 -0.37314748358429384 11103 42-43 0.2194722490613401 11103 44-45 -0.6169203685421891 11103 46-47 0.21859792704250225 11103 48-49 0.7554206484977506 11103 50-51 0.10759898293303394 11103 52-53 -0.7611126227699572 11103 54-55 1.1271612795956436 11103 56-57 -0.5169295962724796 11103 58-59 -0.17273730328276926 11103 60-61 0.9300938145591253 11103 62-63 -0.4515617352363037 11103 64-65 -0.7483043454629623 11103 66-67 -0.7623282601376573 11103 68-69 -0.5732272453693312 11103 70-71 -1.159891484652583 11103 72-73 -0.5536831116846557 11103 74-75 0.07404226475707532 11103 76 -0.4696825425241329 11104 1 -1.3929219894629625 11104 2 -0.21719500587290597 11104 3 0.029002253670679323 11104 4 0.3138520578098962 11104 5 -1.9765274749302861 11104 6 -1.2156430650904966 11104 7 -0.45616812839225673 11104 8 -0.7302244359104009 11104 9 -0.47130245421651296 11104 10-11 -0.0497413403353022 11104 12-13 0.130202320740608 11104 14-15 -0.4514071972794298 11104 16-17 0.23011538625040373 11104 18-19 -0.4807404959722916 11104 20-21 0.1694669700657201 11104 22-23 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62-63 0.40086857713237833 11105 64-65 0.17311322002240104 11105 66-67 -1.3009541181009538 11105 68-69 0.24663548050263984 11105 70-71 -0.3285425562312341 11105 72-73 0.24527332479104302 11105 74-75 0.29448630453855884 11105 76 0.14531955861048118 11106 1 -0.16655237184847493 11106 2 -0.642509317644123 11106 3 0.17791763377621095 11106 4 -0.5209469780835292 11106 5 -1.9854519749628565 11106 6 -0.9097997975509173 11106 7 0.10425138825161184 11106 8 -0.3081672409393903 11106 9 -0.37326323853023524 11106 10-11 -0.1279121435402999 11106 12-13 -0.5849302439131634 11106 14-15 0.0292129577593343 11106 16-17 -0.3580873366992421 11106 18-19 -0.9893067131568429 11106 20-21 -0.4413727146453681 11106 22-23 -0.7277634694635111 11106 24-25 -0.26781529617529287 11106 26-27 -0.715886207596526 11106 28-29 0.5281728247366502 11106 30-31 -0.8164114129799174 11106 32-33 0.08669228137596008 11106 34-35 -0.013265494397501243 11106 36-37 -0.006312317120844568 11106 38-39 -0.1869982909631176 11106 40-41 0.09067533367820246 11106 42-43 -0.8635999628742823 11106 44-45 0.1933630668658779 11106 46-47 -0.12134652531471346 11106 48-49 0.062036515183407914 11106 50-51 -0.4885055605792914 11106 52-53 0.26662559115968065 11106 54-55 0.6167639003049423 11106 56-57 1.0116988042402646 11106 58-59 -0.5506393115437014 11106 60-61 0.8217759111265686 11106 62-63 -0.637432693789215 11106 64-65 0.06119572575196486 11106 66-67 -1.1465683968703146 11106 68-69 -0.13148105536790666 11106 70-71 -0.19464436885711223 11106 72-73 -1.0097791094057769 11106 74-75 -0.3190285228800107 11106 76 -1.1856062001227627 11107 1 -0.6470652666615706 11107 2 -0.5059546957953849 11107 3 0.2344954373114163 11107 4 -0.10548769092038768 11107 5 -0.3341190279909654 11107 6 1.205166878774783 11107 7 0.31621893389055344 11107 8 0.22660528876214414 11107 9 -0.2500380864004157 11107 10-11 -0.046232920126797694 11107 12-13 0.20321087460321507 11107 14-15 0.6671439922420959 11107 16-17 -0.14785841754537898 11107 18-19 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11109 40-41 1.2472063671442193 11109 42-43 1.3382649936346382 11109 44-45 1.0611944099294135 11109 46-47 0.730120377235778 11109 48-49 -0.024835075558154784 11109 50-51 0.8948425154932451 11109 52-53 0.6230681681904962 11109 54-55 1.4907503369848811 11109 56-57 1.065288661235698 11109 58-59 0.9709588013856845 11109 60-61 1.312193130645955 11109 62-63 0.45172402152849145 11109 64-65 0.6523795677098008 11109 66-67 -0.4562027769381274 11109 68-69 0.4507097156773554 11109 70-71 -0.12881803832490135 11109 72-73 0.4559808788000339 11109 74-75 -0.10050427107402982 11109 76 -0.340036393252646 11110 1 0.6201761126487746 11110 2 -1.0145753854505557 11110 3 0.05346095455279709 11110 4 0.11865024011409275 11110 5 -0.5410155797151006 11110 6 -0.018971052259701082 11110 7 0.23001203733883102 11110 8 0.08005356462421531 11110 9 0.2499619135995843 11110 10-11 -0.9858880925405913 11110 12-13 -1.0554098150519593 11110 14-15 0.11973019913864391 11110 16-17 0.18403813417876336 11110 18-19 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0.41721063398617275 21611 40-41 1.2312505292736766 21611 42-43 0.20456868389477023 21611 44-45 0.5438774105343711 21611 46-47 -0.05375742070735612 21611 48-49 0.38926050822889735 21611 50-51 0.5547789946221044 21611 52-53 0.009562421064057247 21611 54-55 0.3941834888300093 21611 56-57 0.4545052371582621 21611 58-59 0.9000271619422442 21611 60-61 -0.14077117667406824 21611 62-63 0.17117350731191294 21611 64-65 -0.1850372501970412 21611 66-67 0.5561989169517645 21611 68-69 0.21008660617947328 21611 70-71 0.16572219156016033 21611 72-73 0.20537591812247769 21611 74-75 -0.43845042957372016 21611 76 0.7922987549627152 21612 1 -0.6321749845299109 21612 2 0.15313621329552518 21612 3 0.28418195768759347 21612 4 0.5388696758507692 21612 5 1.2478558936391302 21612 6 1.6720947784613003 21612 7 -0.3706149219088175 21612 8 0.31093130757091814 21612 9 0.3529399700259148 21612 10-11 0.17594576325878108 21612 12-13 0.84937074921136 21612 14-15 0.1519402304866091 21612 16-17 0.8979096075329949 21612 18-19 0.021197488523831964 21612 20-21 0.32936830903682335 21612 22-23 -0.9969384121678644 21612 24-25 0.20559952429835704 21612 26-27 0.5048166189886558 21612 28-29 0.6568970416961619 21612 30-31 0.4829010392737132 21612 32-33 0.6307717313377665 21612 34-35 0.8981172405146438 21612 36-37 1.2032369571343153 21612 38-39 0.4426808534219049 21612 40-41 1.644495043380271 21612 42-43 1.3566063014496308 21612 44-45 0.8658993541080449 21612 46-47 0.8288444601703873 21612 48-49 0.3290724204546045 21612 50-51 2.3666128504214754 21612 52-53 1.3442802894026151 21612 54-55 1.4580549621842422 21612 56-57 -0.3101499352555308 21612 58-59 0.520560077302747 21612 60-61 0.9357178515390991 21612 62-63 -0.1383876212147257 21612 64-65 -0.17688677997760038 21612 66-67 0.48127728685772553 21612 68-69 0.853660273891073 21612 70-71 1.4636062040993494 21612 72-73 0.9890749776836074 21612 74-75 0.4531639904889744 21612 76 0.9808567486931281 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 13.0 17 25.0 18 40.0 19 90.0 20 148.0 21 318.0 22 525.0 23 894.0 24 1257.0 25 1937.0 26 2685.0 27 3653.0 28 5066.0 29 7463.0 30 11075.0 31 15234.0 32 19620.0 33 25209.0 34 33268.0 35 33168.0 36 15525.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.052367247897976 21.078381581174877 9.316065684780767 27.553185486146383 2 29.257942554031942 14.325940973985668 40.45652051238643 15.95959595959596 3 27.614479586942416 15.610981011765372 41.023051096126174 15.751488305166042 4 23.889625596027425 34.89998024997884 23.314053550771664 17.896340603222075 5 23.823604096718675 28.393758993313206 36.61033208249866 11.172304827469457 6 31.298213933015433 45.79893343867273 13.965745887531389 8.937106740780452 7 31.38109076545439 21.06537257004204 13.724007561436673 33.829529103066896 8 29.791496205174507 44.997883926304205 11.739976864260926 13.47064300426036 9 29.538695934317072 14.500465536213076 11.822926953136022 44.137911576333835 10-11 29.756114076765943 28.47715191802002 26.572110555599444 15.19462344961459 12-13 29.874728437209043 14.376322546059871 28.889484524447706 26.859464492283387 14-15 29.14736337217504 14.081200801286572 15.440002257145274 41.33143356939311 16-17 42.14118443698332 27.49851874841294 13.89103631182462 16.46926050277911 18-19 42.54722437751287 18.0131198419976 14.709176835719829 24.7304789447697 20-21 29.337527861636996 15.835849109838332 14.581722766131536 40.24490026239314 22-23 29.1001275294277 28.79569334251244 14.37076077510806 27.7334183529518 24-25 41.94550487138032 28.382667874283683 14.569278561694276 15.102548692641719 26-27 42.79683377308707 29.781157845724042 14.9298040156336 12.492204365555288 28-29 41.406069225516255 17.437312699998305 15.675916676543125 25.480701397942312 30-31 31.219137805518088 15.305391113945339 39.8983628575782 13.57710822295837 32-33 40.373822282042816 15.359219849150518 18.528582675165705 25.738375193640955 34-35 42.86399521463599 28.17834509923423 15.62522925166613 13.33243043446365 36-37 30.83401517930198 28.260023135739075 28.392630420675452 12.513331264283497 38-39 28.448212623084952 17.895212030584318 29.3476850153768 24.308890330953925 40-41 28.466226510919245 15.874950623553977 29.649850459906325 26.00897240562045 42-43 41.48427999469557 15.25812522395682 28.319428024863086 14.93816675648452 44-45 28.842366616821373 28.380498264819572 29.526281635301753 13.250853483057304 46-47 41.17341670005474 15.967304143694733 18.257819862422338 24.601459293828192 48-49 40.12893942386367 14.638997827497672 16.433428321530343 28.798634427108315 50-51 29.670174646615692 16.031938605648506 29.132974071043648 25.164912676692154 52-53 27.37917219197021 17.304122111559405 40.12442513331264 15.192280563157745 54-55 27.173796504771097 19.13601146642741 29.035115933932616 24.655076094868885 56-57 40.00705357898598 15.728634709251473 18.44228761673673 25.822024095025814 58-59 28.217050764056612 17.883946911044397 37.92942915829628 15.969573166602713 60-61 39.75284259233135 27.573850971983184 19.0164489461953 13.656857489490168 62-63 27.62297348388662 43.24823799608381 15.17157319158301 13.957215328446559 64-65 26.818635832670267 44.34813523167821 14.73841326809889 14.094815667552634 66-67 26.966073383519745 44.36700788912339 14.31469589065833 14.35222283669853 68-69 27.39412577942048 44.03126146206585 14.42739045791835 14.147222300595322 70-71 38.21205879863443 33.27991422847953 14.216065231498462 14.29196174138758 72-73 56.22155572045674 15.092785134063726 14.323361200380338 14.36229794509919 74-75 57.62186777871514 14.04233947762689 14.206550966223785 14.129241777434181 76 57.940476056115486 14.082480277191516 14.071193977630555 13.905849689062446 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 1.5 10 2.0 11 3.0 12 5.0 13 7.5 14 8.0 15 15.5 16 25.0 17 25.0 18 32.0 19 60.0 20 86.5 21 92.0 22 122.5 23 198.0 24 318.0 25 393.0 26 454.5 27 563.5 28 826.5 29 1042.0 30 1170.5 31 1445.5 32 1878.5 33 2165.0 34 2336.0 35 2796.0 36 3347.0 37 3609.0 38 3897.5 39 4374.5 40 5021.0 41 5871.5 42 6264.0 43 6732.0 44 7989.5 45 10852.0 46 12925.0 47 17732.5 48 24192.0 49 15918.5 50 5993.0 51 5543.5 52 4369.5 53 3385.5 54 3126.0 55 2901.5 56 2431.0 57 1959.5 58 1734.0 59 1596.0 60 1313.5 61 1057.5 62 946.0 63 834.0 64 704.5 65 668.0 66 596.0 67 543.0 68 504.0 69 415.0 70 370.5 71 376.0 72 336.5 73 299.0 74 259.5 75 218.0 76 217.5 77 186.0 78 148.5 79 142.0 80 148.5 81 140.5 82 98.5 83 71.0 84 75.5 85 66.0 86 43.0 87 34.0 88 29.0 89 16.5 90 10.0 91 11.0 92 11.0 93 11.0 94 10.5 95 18.5 96 27.0 97 44.0 98 182.0 99 14043.5 100 27784.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002821431594390994 2 0.002821431594390994 3 0.0 4 0.0 5 0.0 6 0.005642863188781988 7 0.0 8 0.0 9 0.0 10-11 5.642863188781988E-4 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.001410715797195497 20-21 0.0 22-23 5.642863188781988E-4 24-25 0.003667861072708292 26-27 0.01833930536354146 28-29 0.014671444290833169 30-31 0.00902858110205118 32-33 0.010439296899246678 34-35 0.00451429055102559 36-37 0.0 38-39 0.0 40-41 0.002821431594390994 42-43 0.0025392884349518946 44-45 0.0 46-47 0.003385717913269193 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0011285726377563976 56-57 0.0 58-59 0.0016928589566345964 60-61 0.0 62-63 0.0011285726377563976 64-65 0.00451429055102559 66-67 0.005078576869903789 68-69 0.0 70-71 0.0 72-73 0.0025392884349518946 74-75 0.0025392884349518946 76 0.005078576869903789 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 177215.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 29.136359788956916 #Duplication Level Percentage of deduplicated Percentage of total 1 64.69961653174265 18.85111305476399 2 11.858465352287253 6.910250260982423 3 8.033466320641438 7.021978952120306 4 5.517682147422241 6.430606889935954 5 3.6119611108959213 5.261969923539204 6 2.285315877135221 3.9951471376576473 7 1.3092148584266181 2.670202860931637 8 0.755316264476895 1.7605733148999805 9 0.4241391331293334 1.11220833450893 >10 1.406050276949297 5.94306351042519 >50 0.04260758414997869 0.868436644753548 >100 0.04067087577952512 2.3203453432271535 >500 0.0019367083704535772 0.360578957763169 >1k 0.007746833481814309 4.927348136444431 >5k 0.0019367083704535772 4.056090060096492 >10k+ 0.0038734167409071544 27.51008661794995 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 27934 15.762774031543605 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT 20818 11.747312586406343 No Hit ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT 7188 4.056090060096492 No Hit TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG 3163 1.784837626611743 No Hit CTTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT 2913 1.6437660468921933 No Hit ATTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT 1357 0.7657365347177157 No Hit CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC 1299 0.7330079282227803 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT 639 0.360578957763169 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC 470 0.26521456987275344 No Hit ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT 378 0.21330022853595915 No Hit ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT 333 0.1879073441864402 RNA PCR Primer, Index 26 (95% over 21bp) ATCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC 308 0.17380018621448523 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT 233 0.13147871229862032 No Hit ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT 233 0.13147871229862032 No Hit CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT 206 0.11624298168890895 No Hit CTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTC 196 0.11060011850012696 No Hit CTTAAACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT 178 0.10044296476031937 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0011285726377563976 0.0 2 0.0 0.0 0.0 0.005078576869903789 0.0 3 0.0 0.0 0.0 0.005078576869903789 0.0 4 0.0 0.0 0.0 0.007335722145416584 0.0 5 0.0 0.0 0.0 0.01410715797195497 0.0 6 0.0 0.0 0.0 0.01410715797195497 0.0 7 0.0 0.0 0.0 0.01410715797195497 0.0 8 0.0 0.0 0.0 0.01410715797195497 0.0 9 0.0 0.0 0.0 0.020314307479615157 0.0 10 0.0 0.0 0.0 0.027085743306153542 0.0 11 0.0 0.0 0.0 0.027085743306153542 0.0 12 0.0 0.0 0.0 0.027085743306153542 0.0 13 0.0 0.0 0.0 0.027085743306153542 0.0 14 0.0 0.0 0.0 0.027085743306153542 0.0 15 0.0 0.0 0.0 0.02765002962503174 0.0 16 0.0 0.0 0.0 0.03216432017605733 0.0 17 0.0 0.0 0.0 0.03216432017605733 0.0 18 0.0 0.0 0.0 0.03329289281381373 0.0 19 0.0 0.0 0.0 0.0457071918291341 0.0 20 0.0 0.0 0.0 0.05304291397455069 0.0 21 0.0 0.0 0.0 0.058685777163332675 0.0 22 0.0 0.0 0.0 0.06037863611996727 0.0 23 0.0 0.0 0.0 0.06320006771435827 0.0 24 0.0 0.0 0.0 0.06432864035211466 0.0 25 0.0 0.0 0.0 0.07222864881640945 0.0 26 0.0 0.0 0.0 0.07730722568631324 0.0 27 0.0 0.0 0.0 0.0931072426149028 0.0 28 0.0 0.0 0.0 0.10665011426797957 0.0 29 0.0 0.0 0.0 0.1405072934006715 0.0 30 0.0 0.0 0.0 0.16815732302570324 0.0 31 0.0 0.0 0.0 0.20765736534717716 0.0 32 0.0 0.0 0.0 0.24941455294416387 0.0 33 0.0 0.0 0.0 0.29850746268656714 0.0 34 0.0 0.0 0.0 0.3735575430973676 0.0 35 0.0 0.0 0.0 0.45368620037807184 0.0 36 0.0 0.0 0.0 0.6037863611996727 0.0 37 0.0 0.0 0.0 0.8678723584346697 0.0 38 0.0 0.0 0.0 1.1251869198431284 0.0 39 0.0 0.0 0.0 1.3548514516265553 0.0 40 0.0 0.0 0.0 1.6939875292723527 0.0 41 0.0 0.0 0.0 2.0844736619360664 0.0 42 0.0 0.0 0.0 2.5099455463702283 0.0 43 0.0 0.0 0.0 2.9043816832660894 0.0 44 0.0 0.0 0.0 3.390232203820218 0.0 45 0.0 0.0 0.0 3.846175549473803 0.0 46 0.0 0.0 0.0 4.378861834494822 0.0 47 0.0 0.0 0.0 4.857940919222414 0.0 48 0.0 0.0 0.0 5.316141410151511 0.0 49 0.0 0.0 0.0 5.741049008266795 0.0 50 0.0 0.0 0.0 6.184578054905058 0.0 51 0.0 0.0 0.0 6.640521400558644 0.0 52 0.0 0.0 0.0 7.076714725051491 0.0 53 0.0 0.0 0.0 7.516858053776486 0.0 54 0.0 0.0 0.0 7.94176565189177 0.0 55 0.0 0.0 0.0 8.367237536325932 0.0 56 0.0 0.0 0.0 8.75320937843862 0.0 57 0.0 0.0 0.0 9.139181220551308 0.0 58 0.0 0.0 0.0 9.532488784809413 0.0 59 0.0 0.0 0.0 9.938774934401716 0.0 60 0.0 0.0 0.0 10.305561041672545 0.0 61 0.0 0.0 0.0 10.67065428998674 0.0 62 0.0 0.0 0.0 11.041954687808595 0.0 63 0.0 0.0 0.0 11.371497898033462 0.0 64 0.0 0.0 0.0 11.756341167508394 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CGTCTGC 25 2.8311497E-6 69.99972 51 GTCGTCT 25 2.8311497E-6 69.99972 49 CGTCGTC 25 2.8311497E-6 69.99972 48 TAACTCG 15 0.002221827 69.99972 36 CTTAAAC 435 0.0 65.99537 1 AGGGGGG 3815 0.0 65.412575 70 ATTATAC 1085 0.0 65.17942 1 ACTCCGA 70 0.0 64.99974 11 CGAATGC 50 1.4551915E-11 62.99975 41 GACGTAC 45 3.8198777E-10 62.221973 26 TTAAACA 730 0.0 61.849068 2 TAAACAC 735 0.0 61.42832 3 CGTACGA 40 1.0602889E-8 61.249752 28 ACGAGTA 40 1.0602889E-8 61.249752 31 GTACGAG 40 1.0602889E-8 61.249752 29 AACACAT 825 0.0 61.09066 5 TCTTGAA 35 2.9616058E-7 59.999756 56 CGTCTAC 35 2.9616058E-7 59.999756 48 GAGACGA 100 0.0 59.49976 24 AAACACA 915 0.0 58.524357 4 >>END_MODULE