FastQCFastQC Report
Wed 25 May 2016
SRR1780085_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780085_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119530
Sequences flagged as poor quality0
Sequence length76
%GC73

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5603046.87526144064252No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG1970916.48874759474609No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT17311.4481720070275244No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG13461.1260771354471681No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC12531.048272400234251No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG6480.5421233163222622No Hit
CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG2620.2191918346858529No Hit
CTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT1950.16313896093031038No Hit
CTTATACACATCTGACGCTGCCGACGACATGGTTAGTGTAGATCTCGGTG1620.13553082908056555No Hit
CTTAATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT1320.11043252739897932No Hit
TATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGT1310.10959591734292645No Hit
CTTTACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG1300.1087593072868736No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1270.10624947711871498No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCACAAT150.002220016270.01
ACAACTA150.002220016270.022
TATCAAT207.899628E-570.058
TCTTATG150.002220016270.048
TCTTAAT252.8254362E-670.01
CGTCTGG150.002220016270.09
TGACGCA150.002220016270.013
ATGTAAG150.002220016270.01
ACGTATC150.002220016270.055
CTATACA352.953293E-760.01
TTACACA352.953293E-760.03
TGGTAGC308.334426E-658.33333249
AAAAAGG11650.057.98283470
AGCCGTA252.3823479E-455.99999653
CGACTAG509.695214E-1055.99999625
TGGTCGA252.3823479E-455.99999649
CGACATG653.6379788E-1253.84615325
GACATGG653.6379788E-1253.84615326
CGTATCA28900.053.04498356
TCTCGGT28900.053.04498343
Produced by FastQC (version 0.11.5)