FastQCFastQC Report
Wed 25 May 2016
SRR1780085_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780085_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119530
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2925024.470844139546557No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2540321.252405253911153No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT98198.214674140383167No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG43183.612482222036309No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT38273.201706684514348No Hit
ATTAAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT19911.6656906216012717No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC12101.0122981678239773No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT6470.5412867062662093RNA PCR Primer, Index 39 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC6320.5287375554254162No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC3240.2710616581611311No Hit
CTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC2660.2225382749100644No Hit
CTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2520.21082573412532418No Hit
CTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG2170.1815443821634736No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC1950.16313896093031038No Hit
CTTTACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC1700.14222370952898855No Hit
TCTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG1600.1338576089684598No Hit
TATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCT1550.1296745586881954RNA PCR Primer, Index 39 (95% over 21bp)
CTAAAACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT1380.11545218773529659No Hit
ATAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT1360.11377896762319083No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT1340.11210574751108508No Hit
CTCTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC1300.1087593072868736No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCGC150.002220016270.034
GTACAAC301.0164513E-770.033
ATCAAGA150.002220016270.037
GACTTAT150.002220016270.049
ATTCCGT150.002220016270.045
AATGCCG500.070.043
TATTCCG150.002220016270.044
TCTCGAA700.070.038
AACGAGC150.002220016270.013
CCAAGAG150.002220016270.020
CCGACTT301.0164513E-770.047
AACTCGT353.6761776E-970.037
GATCTTG150.002220016270.042
ATTATGC150.002220016270.051
CGACTTA150.002220016270.048
CAACTCG353.6761776E-970.036
CGACTAA150.002220016270.048
CTAGGAC150.002220016270.026
TGCCGAC401.3278623E-1070.045
ACTCGTA455.456968E-1269.9999938
Produced by FastQC (version 0.11.5)