FastQCFastQC Report
Wed 25 May 2016
SRR1780084_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780084_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115908
Sequences flagged as poor quality0
Sequence length76
%GC80

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6862959.209890602891946No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG2454521.17627773751596No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT22851.9713911032888152No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG12491.0775787693688097No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC12221.054284432480933No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG6530.5633778513993857No Hit
CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG2640.22776684957034893No Hit
CTTATACACATCTGACGCTGCCGACGACATGGTTAGTGTAGATCTCGGTG2450.21137453842702833No Hit
CTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT2380.2053352659005418No Hit
CTTATACACATCTGACGCTGCCGACGACTAGGTTAGTGTAGATCTCGGTG2100.18117817579459572No Hit
CTTTACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG1810.1561583324705801No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGATAGTGTAGATCTCGGTG1370.11819719087552197No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1340.11560893122131347No Hit
TATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGT1290.11129516513096595No Hit
TCTTAATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG1290.11129516513096595No Hit
CTTAATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT1180.10180487973220141No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGTTTG150.002216428370.0271930
GTTTGTG150.002216428370.0271932
ACTTGGA150.002216428370.0271927
TGGTAAG150.002216428370.0271930
GGTTTGT150.002216428370.0271931
TGATAGT207.88343E-570.0271931
CTTGGAT150.002216428370.0271928
GTAAGTG150.002216428370.0271932
TTGATAG150.002216428370.0271930
TGGATAG150.002216428370.0271930
GCTTGGT150.002216428370.0271927
TTGGTTT150.002216428370.0271929
TTGGATA150.002216428370.0271929
CTTTGAT150.002216428370.0271928
GATAGTG353.6634447E-970.0271932
TTTGTGT150.002216428370.0271933
GGTAAGT150.002216428370.0271931
TTTGATA150.002216428370.0271929
CTTTACA207.9003104E-569.996991
TGCGCGT207.9003104E-569.996995
Produced by FastQC (version 0.11.5)