FastQCFastQC Report
Wed 25 May 2016
SRR1780084_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780084_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115908
Sequences flagged as poor quality0
Sequence length76
%GC64

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3557330.690720226386443No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT3330528.733995927804813No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT102988.884632639679745No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG53074.578631328294855No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT51974.48372847430721No Hit
ATTAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT23542.0309210753356113No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC10850.9360872416054112No Hit
ATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT7010.6047900058667219Illumina PCR Primer Index 3 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC6780.5849466818511233No Hit
CTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC3100.267453497601546No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC3010.2596887186389205No Hit
CTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT2610.22517858991614037No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC2380.2053352659005418No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCAGCCATCTCGTATGCCGT2230.19239396762949926No Hit
CTAAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT2230.19239396762949926No Hit
TCTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG2080.1794526693584567No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC1880.16219760499706662No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG1760.1518445663802326No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG1730.14925630672602408No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACGCCATCTCGTATGCCGT1670.14407978741760707No Hit
TATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCT1640.14149152776339854No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC1590.13717776167305104No Hit
ATAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT1590.13717776167305104No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG1410.12164820374779997No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGACGT1380.11905994409359145No Hit
ATAAAACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT1340.11560893122131347No Hit
ATACCCATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT1170.1009421265141319Illumina PCR Primer Index 3 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCA550.070.0764627
ACTCGCC150.0022102570.07645427
GACTCGC700.070.07645426
ATTAAAC3700.070.0159761
ATCTTAT353.6670826E-970.015971
CTTTACA207.889693E-570.015971
TTATACC207.889693E-570.015972
CTCTAAT207.889693E-570.015971
ATAAAAC401.3278623E-1070.015971
GCCATCA301.0145595E-770.0159734
GCCAACT207.889693E-570.0159734
TCTTTAT1000.070.015971
TTTACAC301.0145595E-770.015972
TCATATA301.0145595E-770.015971
CTTCTAC150.002217837170.015972
TCTTAAT401.3278623E-1070.015971
ACTGCTA150.00222163869.9857653
ACTCGTA252.828312E-669.9857638
AATCTCG500.069.9857636
CACATAT150.00222163869.985767
Produced by FastQC (version 0.11.5)