FastQCFastQC Report
Wed 25 May 2016
SRR1780083_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780083_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429455
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7209916.788487734454133No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG201494.691760487129035No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT17620.4102874573587454No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG15910.37046954861394094No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC12130.2824510134938468No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG5760.1341234820877624No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCGGTAG150.002224085269.99895513
CCGGTAC150.002224085269.99895532
GAGATCG150.002224085269.99895522
GTGGACG252.3895223E-455.9991648
GCCGTAT30050.052.99421354
TGGTCGC30450.052.87277249
TCGGTGG30600.052.8423545
CGGTGGT30650.052.75614546
CTCGGTG30400.052.6143544
TCGCCGT30350.052.58570552
CCGCTAG200.006946298852.5053334
CGGTACG200.006946298852.5053333
AGCCGTC200.006949498352.49921418
ACGCCGT200.006949498352.49921452
GTCTCGG200.006949498352.49921442
TCTCGGT30500.052.4418443
GTCGCCG30600.052.3848451
ATCTCGG30350.052.35506442
GATCTCG30550.052.12687741
CGCCGTA30750.052.0154853
Produced by FastQC (version 0.11.5)