FastQCFastQC Report
Wed 25 May 2016
SRR1780083_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780083_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences429455
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT318257.410555238616386No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG302797.050564087040552No Hit
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT125652.925801306306831No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG54651.2725431069611484No Hit
CTTAAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT49181.1451723696312768No Hit
ATTAAACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT26500.6170611589107124No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC12560.29246370399692634No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT8370.19489818490877972TruSeq Adapter, Index 3 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC6470.15065606408121923No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC4920.11456380761663038No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTCGA150.002222060570.0150222
AACGTAC150.002223087270.0068761
CGTACCG150.002223087270.0068763
ACGTACC150.002223087270.0068762
CGGGAAC150.002224114369.9987257
GGTCACG207.928259E-569.9905769
GCCGTAT455.456968E-1269.9905746
AGGGGGG60950.064.13409470
ATTATAC16000.061.686371
AATCTCG950.058.93942636
CGTCTTC68500.056.0946348
TCCGCGG252.389561E-455.99897413
CACGTAT1000.055.9924639
TAAGACG252.3909363E-455.9924643
CCGTCTT68900.055.87055247
TATGCCG71300.055.8550343
GCCGTCT69300.055.80056446
TCTCGTA71500.055.74773438
CGTATGC72150.055.73053741
ATCTCGT71400.055.72778737
Produced by FastQC (version 0.11.5)