FastQCFastQC Report
Wed 25 May 2016
SRR1780082_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780082_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95829
Sequences flagged as poor quality0
Sequence length76
%GC79

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5644958.90596792202778No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG1930820.14838931847353No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT17181.7927767168602406No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG10711.1176157530601383No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC9360.9767398178004572No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG6020.6282023187135418No Hit
CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG2230.23270617454006615No Hit
CTTATACACATCTGACGCTGCCGACGACATGGTTAGTGTAGATCTCGGTG1830.19096515668534578No Hit
CTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT1680.17531227498982563No Hit
CTTTACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG1520.15861586784793746No Hit
CTTATACACATCTGACGCTGCCGACGACTAGGTTAGTGTAGATCTCGGTG1280.13357125713510523No Hit
TCTTAATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG1220.12731010445689717No Hit
CTTAATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT1170.12209247722505714No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGATAGTGTAGATCTCGGTG1100.11478779910048106No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGCG252.8211489E-670.000012
TGCGGGT252.8211489E-670.000015
ATCTCTG150.002218667470.042
AACAACT207.891635E-570.07
TTACACA301.0142867E-770.03
GTATCAG150.002218667470.057
TCAATAA150.002218667470.060
TCTGTGG150.002218667470.045
AGTGTGG150.002218667470.035
ATAAACA301.0142867E-770.04
GATCTCT150.002218667470.041
CTGTGGT150.002218667470.046
GTCTCGG207.891635E-570.042
CTAATAA150.002218667470.01
TGTGGTC150.002218667470.047
TGTGGGT207.891635E-570.037
GCCGTGG150.002218667470.054
ATCAATA150.002218667470.059
CGCATCT150.002218667470.07
GGGTGTC150.002218667470.040
Produced by FastQC (version 0.11.5)