##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780082_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 95829 Sequences flagged as poor quality 0 Sequence length 76 %GC 79 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 29.661824708595518 32.0 27.0 32.0 27.0 32.0 2 30.378455373634285 32.0 32.0 32.0 27.0 32.0 3 30.251938348516628 32.0 32.0 32.0 27.0 32.0 4 29.635820054472028 32.0 32.0 32.0 27.0 32.0 5 30.369543666322304 32.0 32.0 32.0 27.0 32.0 6 34.87010195243611 37.0 37.0 37.0 32.0 37.0 7 34.53220841290215 37.0 37.0 37.0 27.0 37.0 8 34.60876143964771 37.0 37.0 37.0 32.0 37.0 9 34.91354391676841 37.0 37.0 37.0 32.0 37.0 10-11 34.568277869955864 37.0 37.0 37.0 29.5 37.0 12-13 34.29558380031097 37.0 37.0 37.0 27.0 37.0 14-15 34.01590332780265 37.0 34.5 37.0 27.0 37.0 16-17 34.90980287804318 37.0 37.0 37.0 29.5 37.0 18-19 34.74973650982479 37.0 37.0 37.0 29.5 37.0 20-21 35.00716380218932 37.0 37.0 37.0 32.0 37.0 22-23 35.14944327917436 37.0 37.0 37.0 32.0 37.0 24-25 34.23883688653748 37.0 37.0 37.0 27.0 37.0 26-27 34.60778052572812 37.0 37.0 37.0 29.5 37.0 28-29 34.82340940633837 37.0 37.0 37.0 32.0 37.0 30-31 34.55765478091183 37.0 37.0 37.0 29.5 37.0 32-33 34.67639232382682 37.0 37.0 37.0 29.5 37.0 34-35 33.490404783520646 37.0 34.5 37.0 22.5 37.0 36-37 35.246600715858456 37.0 37.0 37.0 32.0 37.0 38-39 35.64408477600726 37.0 37.0 37.0 34.5 37.0 40-41 35.10425862734663 37.0 37.0 37.0 32.0 37.0 42-43 35.03081530643125 37.0 37.0 37.0 32.0 37.0 44-45 34.83220110822403 37.0 37.0 37.0 32.0 37.0 46-47 34.959422513017984 37.0 37.0 37.0 29.5 37.0 48-49 35.35799183963101 37.0 37.0 37.0 32.0 37.0 50-51 35.67293303697211 37.0 37.0 37.0 34.5 37.0 52-53 35.15437393690845 37.0 37.0 37.0 32.0 37.0 54-55 35.13094157301026 37.0 37.0 37.0 32.0 37.0 56-57 35.10277160358555 37.0 37.0 37.0 32.0 37.0 58-59 34.77332540253994 37.0 37.0 37.0 29.5 37.0 60-61 34.815207296329916 37.0 37.0 37.0 32.0 37.0 62-63 34.23225745859813 37.0 37.0 37.0 27.0 37.0 64-65 33.599140135032194 37.0 34.5 37.0 20.0 37.0 66-67 33.985672395621364 37.0 37.0 37.0 24.5 37.0 68-69 34.33805006835092 37.0 37.0 37.0 27.0 37.0 70-71 34.35122979473854 37.0 37.0 37.0 27.0 37.0 72-73 34.269803504158446 37.0 37.0 37.0 27.0 37.0 74-75 32.20322136305294 37.0 29.5 37.0 20.0 37.0 76 31.51732774003694 37.0 32.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.08948875237135923 11101 2 -0.24063062127941848 11101 3 -1.027071864087592 11101 4 0.025866300752799276 11101 5 -0.9906918919275682 11101 6 -0.03588718011476999 11101 7 -0.7291182342076326 11101 8 0.13885449429666608 11101 9 -0.13007543352516393 11101 10-11 -0.1004915387880132 11101 12-13 -0.6311896054776795 11101 14-15 0.6238412483030658 11101 16-17 0.04835434982419429 11101 18-19 0.015871611483149195 11101 20-21 -0.7746421735097968 11101 22-23 -0.624426079629508 11101 24-25 -0.6280549147798382 11101 26-27 -0.21847520030910772 11101 28-29 -0.1021242634497952 11101 30-31 0.6501624986666101 11101 32-33 -0.4725969647974182 11101 34-35 0.4395161839256829 11101 36-37 -0.18432855108196122 11101 38-39 -0.4952407444850522 11101 40-41 0.22387732771507274 11101 42-43 0.07396809475540778 11101 44-45 -0.2051256562259809 11101 46-47 0.21614751120161202 11101 48-49 0.4150314264871895 11101 50-51 0.16945925127424744 11101 52-53 -0.10422467160753257 11101 54-55 0.42814584581518034 11101 56-57 -0.042943800378907326 11101 58-59 -0.3289797001301835 11101 60-61 -0.25686757705204144 11101 62-63 -0.25758155882046907 11101 64-65 0.2598765154925218 11101 66-67 0.016191237659008095 11101 68-69 0.19755304523055628 11101 70-71 -0.14761809225123557 11101 72-73 -0.5319115904384262 11101 74-75 0.5657579918957154 11101 76 1.9536295017510845 11102 1 0.43454468545517955 11102 2 0.9960464947080432 11102 3 0.5476407795905693 11102 4 0.7322403864372298 11102 5 -0.2571495721783492 11102 6 1.7749801135111483 11102 7 0.36962784729706755 11102 8 0.9930864692182908 11102 9 0.938367617676505 11102 10-11 1.2892681268337185 11102 12-13 0.5242440413352796 11102 14-15 0.3746249473626264 11102 16-17 0.3127221659161421 11102 18-19 0.1159238580871289 11102 20-21 0.8118259560617815 11102 22-23 0.05425730595042211 11102 24-25 0.43934320434242125 11102 26-27 0.8071256356365595 11102 28-29 0.7863292370726782 11102 30-31 1.1423255080709964 11102 32-33 0.45373532360382995 11102 34-35 0.915482746099137 11102 36-37 0.42277698705805733 11102 38-39 0.5272252952223653 11102 40-41 0.5739295743190453 11102 42-43 0.10923455243565172 11102 44-45 0.5952923166057857 11102 46-47 0.3470252541483205 11102 48-49 0.8100157525060041 11102 50-51 0.04876959610183462 11102 52-53 0.6415956000748082 11102 54-55 0.4869232752822654 11102 56-57 0.14801753713415877 11102 58-59 0.46412374814567414 11102 60-61 0.4847729663126401 11102 62-63 0.2985835404480852 11102 64-65 0.9302004444371406 11102 66-67 0.8597126587666395 11102 68-69 1.0627568069860445 11102 70-71 0.9127267353349708 11102 72-73 -0.09957094258054155 11102 74-75 0.5370223597118056 11102 76 1.728446638637184 11103 1 -0.9098696067203065 11103 2 -0.1443876979649552 11103 3 0.5650537149139545 11103 4 -0.8853534624999035 11103 5 -1.244937903250264 11103 6 -0.09996335098455944 11103 7 -0.3006570916445881 11103 8 -0.07679141409242618 11103 9 -0.2538530969322679 11103 10-11 -0.1797504168116859 11103 12-13 -0.7341232133006343 11103 14-15 -1.6915215926645075 11103 16-17 0.22565748929923046 11103 18-19 -0.5551525779146829 11103 20-21 -0.4241532388726341 11103 22-23 -0.5831239694905506 11103 24-25 -0.9731398350062932 11103 26-27 -1.7889847216678234 11103 28-29 -0.7602560184678069 11103 30-31 -1.3006867035979255 11103 32-33 -0.06356454071095641 11103 34-35 -0.9341328124716384 11103 36-37 -0.9816780106805183 11103 38-39 -0.6774332303316797 11103 40-41 -0.8322214885484343 11103 42-43 -0.3337776592332702 11103 44-45 -0.5382445445430122 11103 46-47 -0.35243200501042793 11103 48-49 -0.3487359435591273 11103 50-51 -0.5519766725548862 11103 52-53 -1.0566178779894173 11103 54-55 -0.14944036108137482 11103 56-57 -0.5430724673886829 11103 58-59 -0.7634881921528347 11103 60-61 -0.6915047360844682 11103 62-63 -0.6869433704519636 11103 64-65 -0.3162943542964669 11103 66-67 -0.3036749486508157 11103 68-69 -0.2951129352120532 11103 70-71 0.03586106369318287 11103 72-73 0.41083159021005855 11103 74-75 -1.3261617198901803 11103 76 -0.1858455368490226 11104 1 -0.22944818442004333 11104 2 -0.11892676732404439 11104 3 -0.006280789036878787 11104 4 -0.4994886688212361 11104 5 0.5463263636505253 11104 6 -0.5521143606421504 11104 7 0.034066349970863996 11104 8 -0.40931994789400505 11104 9 0.19193268006510067 11104 10-11 -0.03938606211458762 11104 12-13 -0.2275384898590147 11104 14-15 -0.3292520579849665 11104 16-17 -0.4176699909465924 11104 18-19 0.27144970478943975 11104 20-21 0.06739636604395116 11104 22-23 -0.4738175603597412 11104 24-25 -0.932314549668277 11104 26-27 -1.0219741861103202 11104 28-29 -0.0334122959041494 11104 30-31 0.6296249733116426 11104 32-33 0.32405617921880747 11104 34-35 -0.891112619319756 11104 36-37 0.08899802092615516 11104 38-39 0.07980996545409624 11104 40-41 0.016220126903718324 11104 42-43 -0.3740542176178181 11104 44-45 0.2880285019890323 11104 46-47 -0.05337581537038716 11104 48-49 -0.35575964856352016 11104 50-51 0.32818136080771865 11104 52-53 -0.1754721361105709 11104 54-55 -0.01708741990490381 11104 56-57 -0.08883629530791382 11104 58-59 -0.7638174283249128 11104 60-61 0.10063749394186772 11104 62-63 0.1873981572038801 11104 64-65 -0.695245188361433 11104 66-67 -0.02843350879128792 11104 68-69 0.24724878559567287 11104 70-71 0.15537379415849983 11104 72-73 -0.46142477502260704 11104 74-75 -0.07572273832740706 11104 76 -0.02912911893857384 11105 1 -0.11882483060090365 11105 2 0.5720302124828045 11105 3 0.3262477447647001 11105 4 0.09972116436576783 11105 5 -0.3195647689219072 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-0.33868241399795807 11105 66-67 0.4724444543342585 11105 68-69 0.17503631851928958 11105 70-71 -0.9342881900381599 11105 72-73 0.015309202150355361 11105 74-75 -1.3560124661588375 11105 76 2.4857962541957512 11106 1 0.9587871096976066 11106 2 0.9583841570457032 11106 3 -0.01426398231419057 11106 4 -0.7036903494935061 11106 5 0.04674653171775134 11106 6 -0.1319462934152611 11106 7 1.1319655096347319 11106 8 -1.0458745697427432 11106 9 -0.726134546825655 11106 10-11 -0.8278314402658467 11106 12-13 0.2636379807292144 11106 14-15 -0.5643360915984061 11106 16-17 -0.8491825959886157 11106 18-19 0.025231217394484418 11106 20-21 0.5440770383128637 11106 22-23 0.149711851404966 11106 24-25 -0.9541632891640788 11106 26-27 -1.2894111609002366 11106 28-29 0.23654568728912295 11106 30-31 0.2605073262528137 11106 32-33 -0.3767841569156474 11106 34-35 -0.8659025352861462 11106 36-37 -0.8828507185696424 11106 38-39 -0.04203877404170697 11106 40-41 -0.6096202092307408 11106 42-43 -1.0368693436682435 11106 44-45 -0.5659630946496179 11106 46-47 -0.33089682377375595 11106 48-49 0.4450806875709361 11106 50-51 -0.029801832469594558 11106 52-53 -0.0887074302282258 11106 54-55 0.20770249606148639 11106 56-57 -0.5335841944675792 11106 58-59 -0.9501619661400369 11106 60-61 0.01702404856371942 11106 62-63 -0.16029091842637655 11106 64-65 -1.2420939278572334 11106 66-67 -1.1515427524887727 11106 68-69 -0.49308734885811134 11106 70-71 -0.4729875503793153 11106 72-73 -0.37402981703941407 11106 74-75 -1.960596687907234 11106 76 -2.0577005475100023 11107 1 -0.3912128903023948 11107 2 0.028132056205368627 11107 3 -0.12392784786041133 11107 4 -0.9259592570565331 11107 5 -0.20367363634947466 11107 6 -0.7727025959362734 11107 7 -0.28946306179383896 11107 8 -0.7034375949528311 11107 9 -0.44042026111137034 11107 10-11 -0.5982095915263557 11107 12-13 -0.5878326075060798 11107 14-15 -0.02778146974966944 11107 16-17 -0.9103170497701285 11107 18-19 -0.9270797069752632 11107 20-21 -0.10907422219133878 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0.4298189716835168 11108 64-65 1.5304335446702382 11108 66-67 1.0588968079507879 11108 68-69 1.2854840797133207 11108 70-71 1.331957504565736 11108 72-73 0.4050910620814676 11108 74-75 -0.18092635823690983 11108 76 0.6093324195229677 11109 1 0.7445013954118913 11109 2 -0.2701872715257245 11109 3 -0.6285496965999045 11109 4 -0.21797606377922207 11109 5 0.04674653171775134 11109 6 -1.131946293415261 11109 7 0.5748226524918749 11109 8 0.725554001685822 11109 9 0.31672259603148945 11109 10-11 0.3721685597341491 11109 12-13 -0.4077905906993564 11109 14-15 -0.4000503773126951 11109 16-17 -0.042039738845758734 11109 18-19 -0.38191163974837394 11109 20-21 0.3583627525985804 11109 22-23 0.042568994262111914 11109 24-25 -0.14702043202122184 11109 26-27 0.5677316962426247 11109 28-29 -0.12059716985373825 11109 30-31 0.7390787548242415 11109 32-33 0.2446444145129263 11109 34-35 0.041240321856712114 11109 36-37 -0.2542792899982089 11109 38-39 0.4436755116725877 11109 40-41 0.6332369336264065 11109 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-0.048398691839324215 21611 46-47 -0.00841438817656126 21611 48-49 -0.2279872281199289 21611 50-51 0.2987695961018346 21611 52-53 -0.22067464334297426 21611 54-55 -0.4281288856715406 21611 56-57 -0.015668503601062866 21611 58-59 0.21412374814567414 21611 60-61 0.6331832991492021 21611 62-63 -0.4038506373959265 21611 64-65 0.23472106043316643 21611 66-67 -0.31676523492436814 21611 68-69 -1.0948437891391407 21611 70-71 -1.0421912974519145 21611 72-73 -0.6321094423321512 21611 74-75 -0.5176224490313572 21611 76 0.1430020286024103 21612 1 0.1691319372838116 21612 2 -0.4130444143828669 21612 3 0.197066066946892 21612 4 -0.23275438890237155 21612 5 0.888618452900019 21612 6 0.8089404060921268 21612 7 1.2019162485509867 21612 8 0.3802338046414917 21612 9 -0.13007543352516393 21612 10-11 0.6408877715568195 21612 12-13 0.17915522210852686 21612 14-15 0.06349642071685935 21612 16-17 -0.44302496052063134 21612 18-19 -0.27723183679270846 21612 20-21 0.8546681713177904 21612 22-23 0.09109116174979448 21612 24-25 0.277117499013265 21612 26-27 0.1866972134840026 21612 28-29 0.3633929779295215 21612 30-31 -1.255009915126493 21612 32-33 0.4929202765818914 21612 34-35 0.49124032185671496 21612 36-37 0.6346369661594196 21612 38-39 0.7978627037908055 21612 40-41 -0.3537088791814753 21612 42-43 0.36707154303126543 21612 44-45 0.25177089549815435 21612 46-47 0.5006302698223095 21612 48-49 0.11330728855615746 21612 50-51 0.4625626995501051 21612 52-53 0.757844293909713 21612 54-55 0.221249294091038 21612 56-57 0.3794699927245446 21612 58-59 1.2055030584904998 21612 60-61 0.6655462160514034 21612 62-63 0.45793568255884765 21612 64-65 0.5529799637683865 21612 66-67 -0.026912210616849563 21612 68-69 0.11134614867884096 21612 70-71 0.18858880430048686 21612 72-73 -0.18708400423152227 21612 74-75 0.5398959229302065 21612 76 1.4536295017510845 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 2.0 11 7.0 12 15.0 13 44.0 14 67.0 15 134.0 16 239.0 17 374.0 18 481.0 19 493.0 20 457.0 21 455.0 22 610.0 23 599.0 24 693.0 25 809.0 26 932.0 27 1016.0 28 1117.0 29 1344.0 30 1722.0 31 2257.0 32 3271.0 33 5663.0 34 12408.0 35 17858.0 36 42762.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 65.31008358638826 2.907261893581275 4.133404293063686 27.64925022696679 2 64.07141888154942 1.9054774650679858 28.993311001888785 5.029792651493806 3 63.91802064093333 3.2693652234709742 29.462897452754383 3.349716682841311 4 64.38887961262314 24.57943730171982 7.671355819001503 3.360327266655535 5 64.04115664360475 6.80065533398032 27.758820398835425 1.399367623579501 6 65.43872098847888 27.589121723159128 4.129445650358991 2.8427116380030055 7 64.58274635026974 6.4458566822151955 3.748343403353891 25.223053564161162 8 65.16816412568221 27.558463513132768 2.7402978221623937 4.533074539022635 9 64.14237861190244 4.5414227425935785 1.4745014557179976 29.84169718978597 10-11 65.468177691513 15.861586784793747 14.160640307213892 4.5095952164793545 12-13 64.7773638460174 3.886088762274468 15.819324004215844 15.517223387492304 14-15 78.48459234678438 13.251729643427353 4.030095273873253 4.233582735915015 16-17 78.15953417024075 4.3645451794342005 2.1366183514384995 15.339302298886558 18-19 67.00964227574404 2.0588971908001836 13.565972367157825 17.36548816629795 20-21 78.31381879656878 2.2566944253125456 4.1257070106234215 15.303779767495252 22-23 78.21066691711277 1.9790460090369306 1.5397217961159984 18.270565277734296 24-25 67.74498192082731 14.987190658311464 1.5219898047031937 15.745837616158031 26-27 78.0112388276974 14.522819411761636 2.7340509347427955 4.73189082579817 28-29 67.4484493050048 4.571210919564219 13.399528321576156 14.580811453854823 30-31 76.97280544308552 2.2827357348582877 17.7609884376174 2.983470384438786 32-33 80.78620847986475 2.701743767414194 14.71975539253029 1.7922923601907603 34-35 67.23400258797011 13.540927495095378 17.606023291731017 1.6190466252034896 36-37 78.11026004925492 4.779918186751263 15.502254038485619 1.6075677255082021 38-39 79.62151332060232 2.6244664976155443 16.15481743522316 1.5992027465589749 40-41 78.18510054367675 14.644314351605464 4.9906604472550065 2.179924657462772 42-43 67.76359727845723 15.57686688650499 14.178006428183828 2.4815294068539466 44-45 66.31951872355302 2.989716003067981 16.1063775390411 14.584387734337906 46-47 77.50930046906713 1.835049072040155 5.039732438679516 15.615918020213194 48-49 81.23366221948585 1.6993900561941384 14.138278278382735 2.928669445937273 50-51 91.26882259023887 1.5705057967838547 4.685951016915547 2.474720596061735 52-53 69.04610343532161 1.8668865049881036 14.606378094085237 14.480631965605042 54-55 78.38389214120987 1.838170073777249 3.545377704035313 16.232560080977574 56-57 77.94914873967556 2.327595652650307 2.5874348445399855 17.13582076313414 58-59 67.6352423091372 14.044433777184121 14.701861668823307 3.618462244855366 60-61 65.07111625916998 4.741257865573052 16.67344958206806 13.514176293188909 62-63 65.00276534243288 15.953417024074131 14.810234897577978 4.233582735915015 64-65 64.97855510451126 16.293424608930675 16.937293247205904 1.790727039352166 66-67 64.93978795341654 30.269858496472846 2.9766873982552076 1.8136661518554076 68-69 64.97406839265776 31.574471193480054 1.6482484425382713 1.8032119713239207 70-71 65.12501565304504 31.453228701423384 1.5976541303168175 1.82410151521476 72-73 66.09255075473371 30.54049682505726 1.705129476095316 1.6618229441137031 74-75 72.58416296203536 23.99714065076284 1.6989126124433869 1.7197837747584162 76 91.80285100077224 4.69705507899735 1.9034500031306745 1.5966439170997433 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.5 12 1.0 13 1.5 14 1.0 15 1.5 16 3.0 17 3.0 18 2.5 19 5.5 20 11.5 21 14.0 22 14.5 23 23.5 24 32.0 25 32.0 26 37.0 27 62.5 28 81.5 29 80.0 30 80.0 31 112.0 32 152.5 33 161.0 34 179.0 35 192.0 36 212.5 37 238.0 38 250.0 39 301.0 40 418.0 41 1382.0 42 2268.0 43 7959.0 44 12222.0 45 5975.0 46 1156.0 47 924.5 48 487.5 49 267.0 50 252.0 51 269.0 52 276.0 53 237.0 54 208.0 55 206.5 56 217.0 57 243.5 58 258.0 59 230.0 60 205.0 61 211.5 62 215.0 63 185.5 64 157.5 65 163.5 66 146.5 67 125.0 68 112.0 69 85.0 70 77.0 71 83.0 72 79.0 73 67.0 74 53.0 75 47.0 76 53.0 77 54.0 78 51.0 79 53.0 80 54.5 81 65.5 82 71.0 83 67.0 84 66.5 85 71.0 86 83.5 87 91.0 88 103.5 89 122.0 90 143.0 91 168.0 92 178.0 93 192.0 94 243.0 95 351.5 96 423.0 97 510.5 98 932.0 99 29256.5 100 57247.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.005217627231840049 5 0.0 6 0.005217627231840049 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0010435254463680096 20-21 0.0031305763391040286 22-23 0.0 24-25 5.217627231840048E-4 26-27 5.217627231840048E-4 28-29 0.0010435254463680096 30-31 0.0010435254463680096 32-33 0.0020870508927360192 34-35 0.0010435254463680096 36-37 0.0010435254463680096 38-39 0.0 40-41 0.0 42-43 0.0010435254463680096 44-45 5.217627231840048E-4 46-47 5.217627231840048E-4 48-49 5.217627231840048E-4 50-51 0.0 52-53 0.0010435254463680096 54-55 0.0 56-57 5.217627231840048E-4 58-59 0.0010435254463680096 60-61 0.0 62-63 0.0 64-65 0.0020870508927360192 66-67 0.0010435254463680096 68-69 0.0 70-71 0.0010435254463680096 72-73 5.217627231840048E-4 74-75 0.0031305763391040286 76 0.0031305763391040286 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 95829.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 9.136065282951925 #Duplication Level Percentage of deduplicated Percentage of total 1 82.15876641918904 7.506078535725094 2 6.70474014848658 1.2250988740360433 3 4.0434037692747005 1.1082240240428263 4 2.524271844660194 0.9224764945893206 5 1.3363792118789262 0.6104623861252856 6 0.8680753854940035 0.4758476035438124 7 0.5825242718446602 0.37253858435337944 8 0.33123929183323814 0.24209790355737823 9 0.17133066818960593 0.1408759352596813 >10 1.005139920045688 1.852257667303217 >50 0.1142204454597373 0.7189890325475586 >100 0.09137635636778983 1.2553611119807155 >500 0.022844089091947458 1.6049421365139989 >1k 0.022844089091947458 2.910392469920379 >5k 0.0 0.0 >10k+ 0.022844089091947458 79.0543572405013 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 56449 58.90596792202778 No Hit CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG 19308 20.14838931847353 No Hit TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT 1718 1.7927767168602406 No Hit CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG 1071 1.1176157530601383 No Hit ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC 936 0.9767398178004572 No Hit TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG 602 0.6282023187135418 No Hit CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG 223 0.23270617454006615 No Hit CTTATACACATCTGACGCTGCCGACGACATGGTTAGTGTAGATCTCGGTG 183 0.19096515668534578 No Hit CTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT 168 0.17531227498982563 No Hit CTTTACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG 152 0.15861586784793746 No Hit CTTATACACATCTGACGCTGCCGACGACTAGGTTAGTGTAGATCTCGGTG 128 0.13357125713510523 No Hit TCTTAATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG 122 0.12731010445689717 No Hit CTTAATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT 117 0.12209247722505714 No Hit CTTATACACATCTGACGCTGCCGACGACTTGGATAGTGTAGATCTCGGTG 110 0.11478779910048106 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.006261152678208058 0.0 5 0.0 0.0 0.0 0.010435254463680098 0.0 6 0.0 0.0 0.0 0.010435254463680098 0.0 7 0.0 0.0 0.0 0.016696407141888154 0.0 8 0.0 0.0 0.0 0.016696407141888154 0.0 9 0.0 0.0 0.0 0.016696407141888154 0.0 10 0.0 0.0 0.0 0.025044610712832233 0.0 11 0.0 0.0 0.0 0.025044610712832233 0.0 12 0.0 0.0 0.0 0.025044610712832233 0.0 13 0.0 0.0 0.0 0.025044610712832233 0.0 14 0.0 0.0 0.0 0.025044610712832233 0.0 15 0.0 0.0 0.0 0.0323492888374083 0.0 16 0.0 0.0 0.0 0.0323492888374083 0.0 17 0.0 0.0 0.0 0.0323492888374083 0.0 18 0.0 0.0 0.0 0.034436339730144316 0.0 19 0.0 0.0 0.0 0.04695864508656043 0.0 20 0.0 0.0 0.0 0.06261152678208058 0.0 21 0.0 0.0 0.0 0.07095973035302466 0.0 22 0.0 0.0 0.0 0.07722088303123271 0.0 23 0.0 0.0 0.0 0.09078671383401685 0.0 24 0.0 0.0 0.0 0.09287376472675286 0.0 25 0.0 0.0 0.0 0.09391729017312087 0.0 26 0.0 0.0 0.0 0.09391729017312087 0.0 27 0.0 0.0 0.0 0.09913491740496091 0.0 28 0.0 0.0 0.0 0.10957017186864101 0.0 29 0.0 0.0 0.0 0.1293971553496332 0.0 30 0.0 0.0 0.0 0.13878888436694528 0.0 31 0.0 0.0 0.0 0.14505003704515335 0.0 32 0.0 0.0 0.0 0.15444176606246543 0.0 33 0.0 0.0 0.0 0.16696407141888156 0.0 34 0.0 0.0 0.0 0.1951392584708178 0.0 35 0.0 0.0 0.0 0.2045309874881299 0.0 36 0.0 0.0 0.0 0.24001085266464223 0.0 37 0.0 0.0 0.0 0.29844827766125076 0.0 38 0.0 0.0 0.0 0.3746256352461155 0.0 39 0.0 0.0 0.0 0.4570641455091882 0.0 40 0.0 0.0 0.0 0.530110926754949 0.0 41 0.0 0.0 0.0 0.6031577080007096 0.0 42 0.0 0.0 0.0 0.6929008963883584 0.0 43 0.0 0.0 0.0 0.752381846831335 0.0 44 0.0 0.0 0.0 0.8410815097726158 0.0 45 0.0 0.0 0.0 0.9245635454820565 0.0 46 0.0 0.0 0.0 1.0007409030669212 0.0 47 0.0 0.0 0.0 1.0466560227071138 0.0 48 0.0 0.0 0.0 1.1155287021674023 0.0 49 0.0 0.0 0.0 1.1729226017176428 0.0 50 0.0 0.0 0.0 1.2094459923405232 0.0 51 0.0 0.0 0.0 1.2731010445689719 0.0 52 0.0 0.0 0.0 1.3284078932264762 0.0 53 0.0 0.0 0.0 1.392062945454925 0.0 54 0.0 0.0 0.0 1.4640662012543175 0.0 55 0.0 0.0 0.0 1.528764778929134 0.0 56 0.0 0.0 0.0 1.5830281021402708 0.0 57 0.0 0.0 0.0 1.6696407141888154 0.0 58 0.0 0.0 0.0 1.7134687829362718 0.0 59 0.0 0.0 0.0 1.7771238351647205 0.0 60 0.0 0.0 0.0 1.8825199052478894 0.0 61 0.0 0.0 0.0 1.9420008556908661 0.0 62 0.0 0.0 0.0 1.9858289244383225 0.0 63 0.0 0.0 0.0 2.033831094971251 0.0 64 0.0 0.0 0.0 2.077659163718707 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGCG 25 2.8211489E-6 70.00001 2 TGCGGGT 25 2.8211489E-6 70.00001 5 ATCTCTG 15 0.0022186674 70.0 42 AACAACT 20 7.891635E-5 70.0 7 TTACACA 30 1.0142867E-7 70.0 3 GTATCAG 15 0.0022186674 70.0 57 TCAATAA 15 0.0022186674 70.0 60 TCTGTGG 15 0.0022186674 70.0 45 AGTGTGG 15 0.0022186674 70.0 35 ATAAACA 30 1.0142867E-7 70.0 4 GATCTCT 15 0.0022186674 70.0 41 CTGTGGT 15 0.0022186674 70.0 46 GTCTCGG 20 7.891635E-5 70.0 42 CTAATAA 15 0.0022186674 70.0 1 TGTGGTC 15 0.0022186674 70.0 47 TGTGGGT 20 7.891635E-5 70.0 37 GCCGTGG 15 0.0022186674 70.0 54 ATCAATA 15 0.0022186674 70.0 59 CGCATCT 15 0.0022186674 70.0 7 GGGTGTC 15 0.0022186674 70.0 40 >>END_MODULE