FastQCFastQC Report
Wed 25 May 2016
SRR1780082_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780082_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95829
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2813929.363762535349423No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2448525.550720554320716No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1103911.51947740245646No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG44214.61342599839297No Hit
CTTAAACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT36913.8516524225443236No Hit
ATTAAACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT22782.377150966826326No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC8510.8880401548591762No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC5460.5697648937169333No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT4920.5134145196130608TruSeq Adapter, Index 11 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC3210.3349716682841311No Hit
CTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC2310.24105437811101021No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC1860.1940957330244498No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT1750.18261695311440168Illumina PCR Primer Index 6 (95% over 21bp)
CTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1690.17635580043619364No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC1520.15861586784793746No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG1490.15548529150883345No Hit
TCTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG1480.15444176606246543No Hit
ATAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1380.14400651159878533No Hit
CTAAAACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1260.13148420624236923No Hit
CCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG1260.13148420624236923No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGACGT1160.12104895177868912No Hit
TATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCT1150.12000542633232111No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT1110.11583132454684908Illumina PCR Primer Index 6 (95% over 21bp)
ATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC1100.11478779910048106No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG1080.11270074820774505No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTAGTATGCCGT1030.107483120975905No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC1020.10643959552953698No Hit
ATACCCATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT960.10017844285132892TruSeq Adapter, Index 11 (95% over 21bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGTA252.8211489E-670.0000138
TCGACCA252.8211489E-670.0000129
ACCACAT252.8211489E-670.0000132
CCGTCTA252.8211489E-670.0000147
ACACAAC455.456968E-1270.000016
TCTGCTA500.070.0000153
ATCAGCT455.456968E-1270.0000152
TCTGCAA252.8211489E-670.0000153
TATCCGA252.8211489E-670.0000111
CCACATA455.456968E-1270.0000133
CCCCCAT252.8211489E-670.0000132
ATATCGT252.8211489E-670.0000137
ATATCCG252.8211489E-670.0000110
CATATCG252.8211489E-670.0000136
CATATCC252.8211489E-670.000019
GACCACG252.8211489E-670.0000118
CGACCAC252.8211489E-670.0000130
GCCCCCA252.8211489E-670.0000131
CGTCATA353.6652636E-970.048
CTACAGC150.002218667470.051
Produced by FastQC (version 0.11.5)