FastQCFastQC Report
Wed 25 May 2016
SRR1780081_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780081_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221696
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4906522.131657765588912No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG170907.708754330254042No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT15820.713589780600462No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG11740.5295539838337183No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC9640.4348296766743649No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG5230.23590863163972287No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTGCCG150.002222529170.0000117
GCGCGTC352.9649527E-760.06
CGCGTCT308.357887E-658.3333367
GTGCGCG252.3868142E-456.0000044
CTTTATA1150.054.7826082
TTGATAA200.006944673652.59
AGATCTA200.006944673652.540
GGAGCAT200.006944673652.540
CTCAAGC200.006944673652.512
GGGTCAG200.006944673652.51
GCGGGAA200.006944673652.560
GGCGCGT200.006944673652.55
TCGACAA200.006944673652.531
TAGTCTG200.006944673652.540
ATCGACA200.006944673652.530
CAAAGGG200.006944673652.567
AAAAAGG11600.052.19827770
TCGCCGT25850.051.7214752
GCCGTAT25900.051.62162454
GTCGCCG26300.051.501951
Produced by FastQC (version 0.11.5)