FastQCFastQC Report
Wed 25 May 2016
SRR1780081_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780081_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221696
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2512111.331282476905312No Hit
CTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT215229.707888279445728No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT101464.5765372401847575No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCG37491.6910544168591224No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT29301.3216296189376444No Hit
ATTAAACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGT19420.8759743071593533No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC12230.5516563221709007No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC5250.23681076789838335No Hit
ATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCCGTCTT4730.21335522517321018RNA PCR Primer, Index 30 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACGCCTCTGGATCTCGTATGCC3860.17411229792147806No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTCAC150.002222529170.0000153
CCGAATG150.002222529170.0000115
CGCACTA150.002222529170.0000129
CGACTTA150.002222529170.0000148
ACGATTC207.914529E-570.024
ATTATAC11700.065.213681
AGGGGGG41450.064.68033670
CCGACTT352.9649527E-760.047
CGACTCT601.8189894E-1258.33333628
TAAACAC7100.057.18313
TGACGTC800.056.87545
CGTCTTC48500.056.7938148
CTCGTAT48200.056.78423339
CCGTCTT47800.056.74686447
TCTCGTA48150.056.69782338
ATCTCGT48100.056.6839937
GTATGCC48200.056.63900842
TCGTATG49150.056.6124140
CGTATGC48550.056.59114541
TATGCCG48200.056.5663943