FastQCFastQC Report
Wed 25 May 2016
SRR1780080_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780080_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265299
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6288123.701936305828518No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG217438.195658483446978No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT20690.7798747827922458No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG14910.5620073954293081No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC13280.5005672844601752No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG7500.2826998970972374No Hit
CTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGG2760.10403356213178339No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAACGC252.3876742E-455.9999968
AAAAAGG13900.052.87769770
CTATCGT200.00694617352.537
GACGTAA200.00694617352.558
ATAGAGT200.00694617352.54
CGTATCA30300.050.4785556
GCCGTAT30350.050.39538654
CCGTATC30450.050.22988555
GTCGCCG30900.050.17799451
CGCCGTA30650.050.13050553
TCGCCGT30500.050.03278752
GTAGATC30950.049.87075838
TCGGTGG31100.049.85530545
TGGTCGC31100.049.74276449
CGGTGGT31150.049.6629246
GGTCGCC31300.049.5367450
TAGATCT31150.049.43820639
TCTCGGT31100.049.40514443
GTGGTCG31350.049.34609248
AGATCTC31250.049.2840
Produced by FastQC (version 0.11.5)