FastQCFastQC Report
Wed 25 May 2016
SRR1780080_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780080_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences265299
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3228312.16853437065349No Hit
CTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2739710.326838774364019No Hit
ATTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT109904.142495825464853No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCG46371.7478392304531867No Hit
CTTAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT46021.7346465685886492No Hit
ATTAAACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT25570.9638181825035149No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC12360.4658894304162473No Hit
ATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTCTT7250.27327656719399623TruSeq Adapter, Index 6 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC6260.23596018077716086No Hit
ATCTTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCC3880.14625008009830417No Hit
CTATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGTC3070.11571849121180253No Hit
CTAATACACATCTCCGAGCCCACGAGACACCACAACATCTCGTATGCCGT2730.10290276254339442No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTAACGG150.00222301370.027
TGTTCGG150.00222301370.045
CTCGATC150.00222301370.08
ATTATAC15350.066.807821
AGGGGGG56400.065.0354670
ATCTCGA650.064.6153937
TTAAACA11550.062.4242442
TAAACAC11950.062.3849373
AACACAT13000.061.9230735
AAGCCGT850.061.76470644
TAAGCCG850.061.76470643
AAACACA13650.060.769234
ACTCCGA750.060.6666711
TCGAATG700.060.040
ATTAAAC5100.059.0196081
CTTAAAC7250.058.4137951
CCGACTT601.8189894E-1258.33333247
AGCCGTC850.057.6470645
CGTCTTC61800.057.42718548
GCCGTCT62650.057.37430246
Produced by FastQC (version 0.11.5)