FastQCFastQC Report
Wed 25 May 2016
SRR1780079_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780079_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493348
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5608011.367229622903102No Hit
CTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTG193733.926842715486837No Hit
TCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGT16780.3401250233101178No Hit
CTCTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG14600.29593714781452446No Hit
ATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGGTGGTC12930.26208680282478086No Hit
TCTTTATACACATCTGACGCTGCCGACGACTTGGTTAGTGTAGATCTCGG5710.11573980233020098No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTCA308.374256E-658.33189814
TACGTAA252.3899859E-455.9986244
ACGCGTT200.006947587252.5040333
CGATCGA200.006950373352.4987166
TGGTCGC28950.051.8640149
GCCGTAT28950.051.50132454
TCGCCGT29200.051.41996852
TCGGTGG28950.051.3804345
CGGTGGT29100.051.35612546
CTCGGTG28950.051.13863844
GTCGCCG29450.051.1023151
TCTCGGT29250.050.8534543
CGCCGTA29600.050.72510553
GTGGTCG30000.050.5154248
CGTATCA29450.050.50809556
TAGATCT29600.050.48862539
GGTGGTC29950.050.482947
GTAGATC29750.050.4693438
GATCTCG29450.050.3892541
GTATCAT29650.050.28544257
Produced by FastQC (version 0.11.5)