FastQCFastQC Report
Wed 25 May 2016
SRR1780079_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780079_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences493348
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG287725.831988778711985No Hit
CTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT264325.357678555502404No Hit
ATTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT86381.7508938923437412No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT44110.8940950404177174No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCG42640.8642986289596797No Hit
ATTAAACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGT20290.4112715567915548No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC14230.2884373707808687No Hit
ATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCCGTCTT8160.1654004880935972Illumina PCR Primer Index 10 (95% over 22bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTAAACTTATCTCGTATGCC5990.12141530927458914No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG51750.062.69229570
ATTATAC13100.055.5751721
AAGGGGG58450.055.2066269
CGTATGC59050.055.17909641
TATGCCG58500.055.09961343
TCGTATG60050.055.0761540
CGTCTTC58000.055.03153248
CTCGTAT59300.055.0054839
ATGCCGT58400.054.9542544
GCCGTCT57850.054.9322346
TCTCGTA59500.054.87941738
TGCCGTC58500.054.8603145
ATCTCGT59550.054.83333237
CCGTCTT57700.054.8324247
CTTGAAA58850.054.65297357
GTATGCC59400.054.2647742
TCTTCTG59300.054.06118450
TATCTCG60000.054.0137736
CTGCTTG59200.053.9751554
GTCTTCT59350.053.9566749