FastQCFastQC Report
Wed 25 May 2016
SRR1780078_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780078_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90563
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5840964.49543411768603No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG1033511.411945275664454No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT9491.047889314620761No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5210.5752901295230944No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4190.4626613517661738No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3700.40855537029471195No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2650.2926139814272937No Hit
GTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1980.21863233329284587No Hit
CTTATACACATCTGACGCTGCCGACGAGCAAGAAAGTGTAGATCTCGGTG1440.15900533330388789No Hit
GGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1140.12587922219891126No Hit
CTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGG1010.11152457405342138No Hit
GGGGGGGGGGGGGGGGGGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG960.10600355553592528No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGGG252.8198992E-670.0000116
TTATGCA207.889299E-570.000012
CGCTGAC252.8198992E-670.0000116
TGCACAT207.889299E-570.000015
GAGCGAT207.889299E-570.0000126
AACAACT207.889299E-570.000017
ACGCTGA207.889299E-570.0000115
AGTAGAT207.889299E-570.0000137
GGCGCCG207.889299E-570.0000151
ACGCAGC207.889299E-570.0000115
TATAAAC207.889299E-570.000013
AGAGTAG207.889299E-570.0000135
ACATAAG252.8198992E-670.000018
GACGCAG207.889299E-570.0000114
CTTATGC207.889299E-570.000011
CGCAGCC207.889299E-570.0000116
GACGATC207.889299E-570.0000123
CGAGCGA207.889299E-570.0000125
GGTGGCC207.889299E-570.0000150
GTGGCCG207.889299E-570.0000151
Produced by FastQC (version 0.11.5)