FastQCFastQC Report
Wed 25 May 2016
SRR1780078_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780078_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences90563
Sequences flagged as poor quality0
Sequence length76
%GC53

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT2233124.657972902841117No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1537216.973819330190036No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT1005811.10608084979517No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG39184.326270110309951No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT14131.5602398330444No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT10231.1296003886797035No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT9201.0158674072192837No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT6340.7000651480185065No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCTT5970.6592096109890353RNA PCR Primer, Index 41 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC5560.6139372591455672No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC5320.5874363702615858No Hit
TATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCG4950.5465808332321146No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCGT4340.47922440731866217No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCGT3830.4229100184402018No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGA3750.41407638881220804No Hit
ATTATAAACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGT3580.39530492585272126No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGCCG2990.33015690734626724No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACGT2910.32132327771827346No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTAAGCCGT2540.28046774068880226No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC2420.2672172962468116No Hit
ATATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC2300.2539668518048209No Hit
ATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCAT2210.24402901847332797No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCAAGTATGCCGT2060.22746596292083965No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCAGT1970.2175281295893466No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATATCGTATGCCGT1850.20427768514735598No Hit
CTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1780.19654825922286145No Hit
CTTATACACATCTCCGAGCCCACGAGACAAAAAGACATCTCGTATGCCGT1680.18550622218786922No Hit
ATATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1660.1832978147808708No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGA1650.18219361107737156No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGCCATCTCGTATGCCGT1610.17777679626337467No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCGCGTATGCCGT1470.16231794441438555No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATTCCGT1420.15679692589688946No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGAATGCCGT1410.15569272219339023No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTAGTATGACGT1380.15238011108289257No Hit
ATTATACACATCTCCGAGACCACGAGACAATAAGACATCTCGTATGCCGT1380.15238011108289257No Hit
TTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTC1270.14023387034440113No Hit
TCTTAATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC1210.1336086481234058No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCCT1200.13250444441990658No Hit
ATTATACACATCTCCGAGCCAACGAGACAATAAGACATCTCGTATGCCGT1170.12919183330940892No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGCCATCTCGTATGCCGT1100.12146240738491437No Hit
TATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCCGTCT1070.1181497962744167RNA PCR Primer, Index 41 (95% over 21bp)
CTATTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGCC1060.11704559257091748No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCTCGTATGACG1050.11594138886741825No Hit
CTTATACACATCTCCGAGCCCACGAGACAATAAGAAATCTCGTATGCCGT1040.11483718516391904No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGACGT1000.11042037034992215No Hit
ATTATACACATCTCCGAGCCCACGAGACAATAAGAAATCTCGTATGCCGT990.10931616664642294No Hit
TCTTATACACATCTCCGAGCCCACGAGACAATAAGACATCACGTATGCCG950.10489935183242605No Hit
ATTATACACATCTCAGAGCCCACGAGACAATAAGACATCTCGTATGCCGT920.10158674072192839No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTTTA207.889299E-570.0000155
CTATACC207.889299E-570.0000142
CTTTACA252.8198992E-670.000011
TATGCTA207.889299E-570.0000153
TCGGCTT207.889299E-570.0000153
GAAGCCA252.8198992E-670.0000131
CGAAGCC252.8198992E-670.0000130
TCTATAC207.889299E-570.000011
TACATGA252.8198992E-670.0000155
ACCGAGA252.8198992E-670.0000113
GTATACC207.889299E-570.0000142
CCTCTTC252.8198992E-670.0000148
TACCGTC252.8198992E-670.0000145
ACTTATG207.889299E-570.0000150
ATTAAAC207.889299E-570.000011
GGCTTGA207.889299E-570.0000155
ATGCTAG500.070.0000154
TTAAGAA207.889299E-570.0000152
TACTGCA207.889299E-570.0000152
GACGACA207.889299E-570.0000146
Produced by FastQC (version 0.11.5)