FastQCFastQC Report
Wed 25 May 2016
SRR1780077_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780077_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107769
Sequences flagged as poor quality0
Sequence length76
%GC69

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4187938.859969007785175No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG77667.2061539032560376No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT7100.6588165427905984No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5320.49364845178112443No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4200.3897224619324667No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2430.2254822815466414No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1100.10207016860136031No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCATA150.002219535669.9990752
AGGAGAC150.002219535669.9990712
GCGCGTG150.002219535669.999076
AGCCCGG150.002219535669.9990742
CCCACCG150.002219535669.999079
TGACAAG150.002219535669.9990720
CGGCTTA150.002219535669.9990715
CCGGTAA150.002219535669.9990745
CCCGGTA150.002219535669.9990744
GCCCGGT150.002219535669.9990743
AAAAAGG8000.060.4022470
ACCCAAG308.329509E-658.33255812
TCTTTAT553.45608E-1157.2719651
CTTTATA553.45608E-1157.2719652
CACAGCG252.3814428E-455.9992567
GCCGTAT10700.052.9899554
CGTATCA10650.052.91009556
TCGCCGT10750.052.7434952
CCGTATC10750.052.7434955
CGCCGTA10750.052.7434953
Produced by FastQC (version 0.11.5)