FastQCFastQC Report
Wed 25 May 2016
SRR1780077_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780077_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107769
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT1591414.76676966474589No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1152910.69788158004621No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT1138210.561478718369846No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG38883.6077165047462625No Hit
ATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCTT8790.8156334381872339Illumina PCR Primer Index 5 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC5800.5381881617162635No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC5140.47694606055544736No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC4810.4463250099750392No Hit
TATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCG3540.3284803607716505No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT2740.25424751087975206No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATATCGTATGCCGT2710.2514637790088059No Hit
ATTATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT2580.23940094090137237No Hit
CTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC2160.20042869470812572No Hit
ATTATAAACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGT2140.19857287346082825No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGACGT1880.17444719724596128No Hit
CTTATACACATCTCCGAGCCCACGAGACAACCATTGATCTCGTATGCCGT1800.16702391225677143No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCGT1740.16145644851487906No Hit
ATATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC1650.15310525290204047No Hit
CTATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC1570.14568196791285062No Hit
ATATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCC1530.14197032541825572No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACCATTGCTCTCGTATGCCG1480.13733077230001206No Hit
ATTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCAGT1440.13361912980541715No Hit
ATTATACACATCTCCGAGACCACGAGACCACCATTGATCTCGTATGCCGT1350.12526793419257856No Hit
TATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTCT1330.12341211294528111Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACCACAATTGATCTCGTATGCCGT1210.11227718546149634No Hit
TTATACACATCTCCGAGCCCACGAGACCACCATTGATCTCGTATGCCGTC1180.10949345359055015No Hit
CTTATACACATCTCCGAGCCCACGAGACCACCATTGATATCGTATGCCGT1140.10578181109595523No Hit
ATTATACACATCTCCGAGCCCACGAGACCACAATTGATCTCGTATGCCGT1100.10207016860136031No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAACGAG150.002213002970.05109420
CTATATC150.002217084970.018572
TGCGATT150.002217084970.0185762
TTGAACC150.002217084970.0185730
GATTACC150.002217084970.0185765
ATCTCGA401.3278623E-1069.9860837
ACCGGGC150.002221172469.9860869
CGAACCT150.002221172469.9860853
TATGCGA150.002221172469.9860860
AATGCAG150.002221172469.9860843
GTGTCAC150.002221172469.986087
TCTCGAA401.3278623E-1069.9860838
AGTCTCC150.002221172469.9860836
GAACCTT150.002221172469.9860854
TCAAGGC150.002221172469.9860850
TGCACGG150.002221172469.9860847
GTATGAC1150.066.9432142
TCCGAGA800.065.61194613
AGGGGGG39250.064.4585270
TGACGTC1250.064.3871945
Produced by FastQC (version 0.11.5)