FastQCFastQC Report
Wed 25 May 2016
SRR1780076_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780076_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156330
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5338834.150834772596426No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG51613.3013497089490182No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT6120.39147956246401844No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5020.3211155888185249No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2890.18486534894134204No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAAACAG207.907238E-570.037
CTCGCAG150.002221299870.046
TTTGCGA150.002221299870.042
ATTGGAC252.3846285E-456.0000043
TATACGC252.3846285E-456.0000043
TCAGTTG252.3846285E-456.00000432
AAAAAGG5700.053.42105570
GTACAAA200.006940860852.51
GCTATCT200.006940860852.534
TGGTTTA200.006940860852.521
ATCCGTC200.006940860852.530
ACGTCTG200.006940860852.58
CACGTCT200.006940860852.57
TTTTACG200.006940860852.52
GCCGTAT7850.052.16560454
TCGCCGT7800.051.60256652
CGTATCA7950.051.50943456
GTATCAT7950.051.50943457
CGGTGGT8400.051.24999646
GTCGCCG8000.051.18750451
Produced by FastQC (version 0.11.5)