FastQCFastQC Report
Wed 25 May 2016
SRR1780076_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780076_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences156330
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2578216.492036077528304No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT106746.827864133563616No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG78144.998400818780784No Hit
TCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG40612.597709972494083No Hit
ATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCTT10770.6889272692381501TruSeq Adapter, Index 8 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC8870.5673894965777522No Hit
TATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCG6940.4439327064542954No Hit
TCTTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC6240.39915563231625406No Hit
ATTATAAACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT5770.3690910253949978No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGACGT4360.2788972046312288No Hit
ATATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC4100.2622657199513849No Hit
CTCTTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC3450.2206870082517751No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCAGT3450.2206870082517751No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATATCGTATGCCGT2910.18614469391671465No Hit
ATTATACACATCTCCGAGACCACGAGACAGATTTAAATCTCGTATGCCGT2900.18550502142902833No Hit
ATTATACACATCTCCGAGCCAACGAGACAGATTTAAATCTCGTATGCCGT2640.16887353674918443No Hit
CTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC2550.16311648436000767No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT2220.1420072922663596No Hit
ATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGA2180.1394486023156144No Hit
CTATTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCC2160.1381692573402418No Hit
CTTATACACATCTCCGAGCCCACGAGACAGATATAAATCTCGTATGCCGT2080.13305187743875138No Hit
ATTATACACATATCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT2060.13177253246337875No Hit
TTATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC2010.12857417002494723No Hit
ATTATACACATCACCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT1890.12089810017271156No Hit
ATATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTC1890.12089810017271156No Hit
TATACACATCTCCGAGCCCACGAGACAGATTTAAATCTCGTATGCCGTCT1810.11578072027122115No Hit
ATTATACACATCTACGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT1710.10938399539435809No Hit
ATTATACACATCTCCGAGCACACGAGACAGATTTAAATCTCGTATGCCGT1690.10810465041898548No Hit
ATTATACACATCTCAGAGCCCACGAGACAGATTTAAATCTCGTATGCCGT1670.10682530544361286No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTT207.910995E-569.99328650
ACGTTCC207.910995E-569.99328652
TCCGCCC150.002222145769.9932810
CCGTCAA150.002222145769.9932847
ATATACG150.002222145769.9932810
TAGGGCG150.002222145769.993287
CGTCAAC150.002222145769.9932848
AGGGGGG46000.065.35242570
ATTATAC35100.064.310071
AATCACG401.0599251E-861.24412536
AACTCGT401.0599251E-861.24412537
TGGTTCC352.9612056E-759.99424413
GTATGAC1700.059.70015342
TGACGTC1950.059.22508645
CGTATGA1800.058.32773241
TATGACG1700.057.6415343
CGACTTC553.6379788E-1157.2672348
CCGTCTA800.056.86954547
CACCGAG800.056.86954512
ATATCGT1300.056.53303537
Produced by FastQC (version 0.11.5)