FastQCFastQC Report
Wed 25 May 2016
SRR1780075_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780075_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108940
Sequences flagged as poor quality0
Sequence length76
%GC75

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5097046.787222324215165No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG90768.331191481549476No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT8280.7600514044428125No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5150.47273728657976866No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4860.44611712869469433No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2250.20653570772902513No Hit
CTTATACACATCTGACGCTGCCGACGAGCAAGAAAGTGTAGATCTCGGTG1490.13677253534055445No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTGTGC401.3278623E-1070.01
CAAACCA150.002219485470.038
TTGTGCA207.8964826E-570.02
GGGCTCG150.002219485470.041
GCGCGTG207.8964826E-570.06
ATGGACT150.002219485470.040
CATGAAG150.002219485470.026
GGCAACC150.002219485470.020
ACACAGC150.002219485470.06
TACGCGT150.002219485470.05
TTCCATA150.002219485470.016
GTTCCAT150.002219485470.015
ACGTCTG301.01559635E-770.08
TAGATTA150.002219485470.05
GTCCAGC150.002219485470.010
CACGTCT301.01559635E-770.07
CGCGTGG150.002219485470.07
TGTACGC150.002219485470.03
GATTACT150.002219485470.07
TGCACGT150.002219485470.05
Produced by FastQC (version 0.11.5)