##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1780075_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 108940 Sequences flagged as poor quality 0 Sequence length 76 %GC 75 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.629456581604554 32.0 27.0 32.0 22.0 32.0 2 29.249935744446486 32.0 32.0 32.0 13.0 32.0 3 29.139205067009364 32.0 32.0 32.0 13.0 32.0 4 28.51909307875895 32.0 27.0 32.0 13.0 32.0 5 29.24958692858454 32.0 32.0 32.0 13.0 32.0 6 33.338645125757296 37.0 37.0 37.0 13.0 37.0 7 32.975729759500645 37.0 32.0 37.0 13.0 37.0 8 33.18792913530384 37.0 37.0 37.0 13.0 37.0 9 33.342986965302 37.0 37.0 37.0 22.0 37.0 10-11 33.054406095098216 37.0 34.5 37.0 13.0 37.0 12-13 32.780287314117864 37.0 32.0 37.0 13.0 37.0 14-15 33.35127134202313 37.0 34.5 37.0 20.0 37.0 16-17 33.700697631723884 37.0 37.0 37.0 20.0 37.0 18-19 33.20304295942721 37.0 34.5 37.0 17.5 37.0 20-21 33.62755645309345 37.0 37.0 37.0 20.0 37.0 22-23 33.732462823572604 37.0 37.0 37.0 20.0 37.0 24-25 33.0408481733064 37.0 34.5 37.0 13.0 37.0 26-27 33.59828804846705 37.0 37.0 37.0 20.0 37.0 28-29 33.491743161373236 37.0 37.0 37.0 22.0 37.0 30-31 33.11666972645493 37.0 34.5 37.0 17.5 37.0 32-33 33.43725445199192 37.0 37.0 37.0 17.5 37.0 34-35 33.58035615935377 37.0 37.0 37.0 22.0 37.0 36-37 34.225344226179544 37.0 37.0 37.0 24.5 37.0 38-39 34.28517532586745 37.0 37.0 37.0 27.0 37.0 40-41 33.755934459335414 37.0 37.0 37.0 24.5 37.0 42-43 33.45815127593171 37.0 37.0 37.0 22.0 37.0 44-45 33.34096750504865 37.0 37.0 37.0 17.5 37.0 46-47 33.67909858637782 37.0 34.5 37.0 20.0 37.0 48-49 34.20034422617955 37.0 37.0 37.0 24.5 37.0 50-51 34.501193317422434 37.0 37.0 37.0 27.0 37.0 52-53 33.4831558656141 37.0 37.0 37.0 17.5 37.0 54-55 33.663732329722784 37.0 37.0 37.0 20.0 37.0 56-57 33.48468881953369 37.0 37.0 37.0 17.5 37.0 58-59 33.05581971727557 37.0 34.5 37.0 13.0 37.0 60-61 32.883642371947865 37.0 34.5 37.0 13.0 37.0 62-63 32.41665595740775 37.0 32.0 37.0 13.0 37.0 64-65 32.31752340738021 37.0 32.0 37.0 13.0 37.0 66-67 32.48426197907105 37.0 32.0 37.0 13.0 37.0 68-69 32.646029924729206 37.0 32.0 37.0 13.0 37.0 70-71 32.577372865797685 37.0 32.0 37.0 13.0 37.0 72-73 32.27493115476409 37.0 32.0 37.0 13.0 37.0 74-75 31.799990820635212 37.0 32.0 37.0 13.0 37.0 76 33.40305672847439 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -0.747418930803935 11101 2 0.9180980150193996 11101 3 0.6047159778228917 11101 4 -0.9350207217918154 11101 5 0.0769983333604749 11101 6 -1.053319939230363 11101 7 1.7018121514850435 11101 8 1.230265109128574 11101 9 0.4420392098809529 11101 10-11 0.3162802869477659 11101 12-13 1.5973171799001378 11101 14-15 0.1963731686175052 11101 16-17 0.31810361758025607 11101 18-19 0.7494996253676689 11101 20-21 -0.4958841318404126 11101 22-23 0.33073318211576463 11101 24-25 0.0807482284822072 11101 26-27 0.37910954836124233 11101 28-29 0.10920854012779557 11101 30-31 1.0964694859895516 11101 32-33 0.5538643291011596 11101 34-35 0.4389049618607004 11101 36-37 0.30711794673148773 11101 38-39 -1.4270921247060784 11101 40-41 0.020164177216997814 11101 42-43 -0.8918496651000183 11101 44-45 0.8306201611244077 11101 46-47 -0.3111708945020979 11101 48-49 -0.25856008176246803 11101 50-51 0.22658261836780724 11101 52-53 0.986308698030328 11101 54-55 0.30374514928723784 11101 56-57 0.2211324076250989 11101 58-59 -0.004725241944264269 11101 60-61 0.02788624972265552 11101 62-63 0.021176050248250533 11101 64-65 1.0179534596984467 11101 66-67 0.40953852172421534 11101 68-69 1.0910369863331866 11101 70-71 0.6503610547106078 11101 72-73 -1.2275885136849354 11101 74-75 1.6676669389575949 11101 76 -0.7380474805386754 11102 1 -0.26029949286482434 11102 2 -0.37083641121011013 11102 3 0.6667768677526347 11102 4 0.29975679577258774 11102 5 -1.8108822287004145 11102 6 -0.6446548338439442 11102 7 -0.21826981572807114 11102 8 -0.7676857105435602 11102 9 -0.39139170346799546 11102 10-11 -1.1869398535674591 11102 12-13 -0.5609088154160133 11102 14-15 -0.08377905620684345 11102 16-17 -0.11178514120194194 11102 18-19 -0.13589264629509046 11102 20-21 -0.8303396353532904 11102 22-23 -0.4762047569943064 11102 24-25 0.06889225658524367 11102 26-27 -1.2922897490626468 11102 28-29 -0.8430748322375408 11102 30-31 0.4037001651464607 11102 32-33 -1.2009658816716708 11102 34-35 -1.335246092003473 11102 36-37 -0.6659499689593744 11102 38-39 0.5592954631159301 11102 40-41 -0.1300114668111121 11102 42-43 0.4904688360709457 11102 44-45 0.669905875410123 11102 46-47 -1.9433430256496393 11102 48-49 0.7472947191742989 11102 50-51 0.5115650539649934 11102 52-53 1.1187735692247145 11102 54-55 -1.0658391598462487 11102 56-57 0.2644579345571856 11102 58-59 0.904378973512415 11102 60-61 0.3712703246641098 11102 62-63 -0.22121270853395458 11102 64-65 0.4007110709396713 11102 66-67 -1.3291909864725042 11102 68-69 0.021218485162229683 11102 70-71 -1.3145101396687835 11102 72-73 -1.8631271553711173 11102 74-75 -0.7035274404335077 11102 76 -1.4271575507962844 11103 1 -1.5136526970377027 11103 2 -1.9644777425563582 11103 3 -1.7957169226100085 11103 4 -2.9101289469000378 11103 5 -1.397568766206625 11103 6 -4.706999592910016 11103 7 -2.9231878485149565 11103 8 -2.7507954969320316 11103 9 -2.537939145097404 11103 10-11 -2.924520578853098 11103 12-13 -3.5360702659873056 11103 14-15 -0.594211246966907 11103 16-17 -0.5522968153201759 11103 18-19 -2.6144073010392503 11103 20-21 -1.6065443050005825 11103 22-23 -1.5623945235119407 11103 24-25 -2.342682507448533 11103 26-27 -1.0740830923314064 11103 28-29 -1.0828910269717653 11103 30-31 -1.1916798646598004 11103 32-33 -0.9626399999031676 11103 34-35 -1.8330106225548874 11103 36-37 -0.8400682004546596 11103 38-39 -0.9295271896411492 11103 40-41 -1.1286453465925277 11103 42-43 -1.8745336477839984 11103 44-45 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11104 24-25 -1.4779791357029097 11104 26-27 -0.041539344998923866 11104 28-29 0.06318238318614533 11104 30-31 -0.2806683409722197 11104 32-33 -0.5677402986654414 11104 34-35 -0.43672381077512057 11104 36-37 -0.24318386413370519 11104 38-39 -0.44105087722117275 11104 40-41 -0.7964857221793835 11104 42-43 -1.2092540916593748 11104 44-45 -0.06953074430818873 11104 46-47 -0.36310751422040966 11104 48-49 -0.9547371441367147 11104 50-51 -0.08818096312113255 11104 52-53 -0.735648042412322 11104 54-55 -1.5150425770709077 11104 56-57 0.2553376390134261 11104 58-59 0.04268421479617501 11104 60-61 -0.6152224021888131 11104 62-63 -0.4063893421058786 11104 64-65 -0.24198114794742764 11104 66-67 -1.1001445768673364 11104 68-69 -0.8494811223187249 11104 70-71 -1.1886731505207777 11104 72-73 -1.6332726183127022 11104 74-75 -0.12005941919129981 11104 76 0.6266406482339661 11105 1 0.08988265649765026 11105 2 1.1680980150193996 11105 3 1.4896366127435279 11105 4 1.2514872147161213 11105 5 1.9004110317731744 11105 6 0.968505457595036 11105 7 1.6879232625961578 11105 8 0.3969317757952382 11105 9 1.3686265114682499 11105 10-11 1.3033834615509363 11105 12-13 0.9266822592652275 11105 14-15 0.8729604702048093 11105 16-17 1.3012385382151805 11105 18-19 1.271325022193075 11105 20-21 0.9168142808580058 11105 22-23 1.619423658306232 11105 24-25 0.6263831491171246 11105 26-27 0.7838714531231474 11105 28-29 1.4941291750484353 11105 30-31 0.15301710503716492 11105 32-33 1.0310468687837044 11105 34-35 1.3248176602733963 11105 36-37 1.1801338197473612 11105 38-39 0.6790586689447125 11105 40-41 1.9477435422963651 11105 42-43 0.9266027158523613 11105 44-45 0.8157392087434516 11105 46-47 0.758273549942345 11105 48-49 0.3475907118883228 11105 50-51 0.9339238882090726 11105 52-53 1.1301579043795371 11105 54-55 0.6559276889697827 11105 56-57 1.3441482806409724 11105 58-59 0.38217951996049493 11105 60-61 1.0893941862305923 11105 62-63 1.2751443042165036 11105 64-65 1.3264852057301937 11105 66-67 1.7012051883908796 11105 68-69 1.1892512720474713 11105 70-71 1.537265816615367 11105 72-73 1.0035622799658555 11105 74-75 1.0932621770528357 11105 76 0.9464763289851348 11106 1 0.5277911532296784 11106 2 -1.515725514392365 11106 3 -0.5969646944460152 11106 4 -1.353087948682571 11106 5 -1.2727915826059117 11106 6 -0.9734880064572522 11106 7 -1.7908349073384855 11106 8 -1.777087832047897 11106 9 -0.605229697682077 11106 10-11 -0.9127113096908914 11106 12-13 -0.9095105511922945 11106 14-15 -1.0714839742396336 11106 16-17 0.5896372310256339 11106 18-19 -0.4243449124474523 11106 20-21 0.2766999017730356 11106 22-23 -0.30214496914474864 11106 24-25 -0.316835805131241 11106 26-27 0.7703910609662827 11106 28-29 -1.0924721321411113 11106 30-31 -0.8347279930020477 11106 32-33 -0.1830054187979968 11106 34-35 -0.08367907175275491 11106 36-37 0.2062776105970343 11106 38-39 -0.27110472974810307 11106 40-41 -0.6310963269846894 11106 42-43 -2.28050512728489 11106 44-45 -1.756564712825174 11106 46-47 -1.0795532474432719 11106 48-49 -0.8709550397456667 11106 50-51 0.6063095091241024 11106 52-53 -1.0005610498688284 11106 54-55 -0.21253636331780257 11106 56-57 -0.5341196932152386 11106 58-59 -1.9180655780787177 11106 60-61 -1.4490045065798647 11106 62-63 -1.2256726892475527 11106 64-65 -1.738874271393989 11106 66-67 -0.9911967723934296 11106 68-69 -0.9651604926584092 11106 70-71 -1.5166557520120776 11106 72-73 -1.501748177550482 11106 74-75 -1.3491397837314807 11106 76 -1.404013867093294 11107 1 1.0772563938713908 11107 2 1.0355222574436418 11107 3 0.24973762284453827 11107 4 1.0292649924939 11107 5 0.6630372943994338 11107 6 2.111182225271797 11107 7 0.6071151817880747 11107 8 1.5370832909467538 11107 9 -0.1591512663095287 11107 10-11 0.7724491181165973 11107 12-13 1.3805964006793587 11107 14-15 1.117909965154304 11107 16-17 0.8037637907404331 11107 18-19 1.5446836080516633 11107 20-21 0.07527387681760445 11107 22-23 1.2179085067910833 11107 24-25 0.9603477955817681 11107 26-27 0.2592502410019364 11107 28-29 1.0004423063615633 11107 30-31 0.9901383171583831 11107 32-33 1.3555418182786525 11107 34-35 0.9548681653239015 11107 36-37 0.47811361772716054 11107 38-39 0.3053212952073352 11107 40-41 0.7955970776498944 11107 42-43 0.6254663522159944 11107 44-45 1.1396028451070919 11107 46-47 1.2551169842857774 11107 48-49 -0.09622241942480514 11107 50-51 -0.1254195461343599 11107 52-53 0.5190467932684228 11107 54-55 0.5145135475556373 11107 56-57 1.1118250483177405 11107 58-59 1.5772552775362598 11107 60-61 1.1020204488568552 11107 62-63 0.39888167795387375 11107 64-65 1.36183874108373 11107 66-67 1.2637051883908867 11107 68-69 1.340766423562627 11107 70-71 0.20014460449416305 11107 72-73 0.6658097547133295 11107 74-75 1.1936409649316175 11107 76 0.12450663201543932 11108 1 -0.033133216518219655 11108 2 -0.7913175693961847 11108 3 0.1956250687319816 11108 4 -0.5876181243892162 11108 5 0.14355677491891683 11108 6 -0.4883848742952992 11108 7 0.23265630732920073 11108 8 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48-49 0.8882109554195239 11109 50-51 0.27049162032474783 11109 52-53 0.8489554690675121 11109 54-55 0.23317274224223183 11109 56-57 -0.07632356106374516 11109 58-59 0.5753872825175748 11109 60-61 1.247308950309737 11109 62-63 1.0133482615985372 11109 64-65 0.3793771387591107 11109 66-67 -0.5673450399196227 11109 68-69 -0.2662281800073245 11109 70-71 -0.20873874959467287 11109 72-73 0.8078811536340424 11109 74-75 2.0062187980573967 11109 76 -0.3588889678185154 11110 1 -0.6045617879467926 11110 2 -0.2793657530965419 11110 3 -0.11060493315019215 11110 4 0.2394099200301305 11110 5 -1.0102169875504998 11110 6 -0.6299244940957891 11110 7 -0.3579704572106053 11110 8 -1.5505319923206997 11110 9 -0.13741213587474022 11110 10-11 -1.392570644729254 11110 12-13 -0.9141993832468529 11110 14-15 1.08431312720964 11110 16-17 -0.05262619608433994 11110 18-19 0.021626954560225897 11110 20-21 0.21361379776621447 11110 22-23 0.1411627887410205 11110 24-25 -1.1378076721389156 11110 26-27 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-1.4156197061238842 21611 68-69 0.03418798090822861 21611 70-71 -0.8451181496293572 21611 72-73 -0.11660298023105042 21611 74-75 0.625171459753254 21611 76 0.7054425737108758 21612 1 0.8121048787198752 21612 2 0.5430980150193996 21612 3 0.497573120680034 21612 4 -0.13740167417276794 21612 5 0.5948554762176173 21612 6 0.37525148934106767 21612 7 -0.4946164199435259 21612 8 1.2838365377000045 21612 9 1.5908487336904713 21612 10-11 1.0246136202811016 21612 12-13 0.18362670370967038 21612 14-15 -0.041722069477728496 21612 16-17 -0.14915828718164903 21612 18-19 -0.2296670412989883 21612 20-21 0.2511396776833976 21612 22-23 0.38728080116338504 21612 24-25 0.083724418958397 21612 26-27 0.02494288169457093 21612 28-29 -0.5276962217769636 21612 30-31 -0.3529352759152147 21612 32-33 -0.44315948042265063 21612 34-35 0.007357342813079981 21612 36-37 0.09878461339815914 21612 38-39 0.7663602562463012 21612 40-41 -0.063169156116345 21612 42-43 0.4771979539476021 21612 44-45 0.16990587541012303 21612 46-47 0.6560910102598072 21612 48-49 -0.020464843667234334 21612 50-51 -0.20496500067981316 21612 52-53 -0.8380960638744313 21612 54-55 -0.33613580309371116 21612 56-57 0.8580371695298581 21612 58-59 -0.5731776228966439 21612 60-61 0.3195529163893198 21612 62-63 -0.22882394975175657 21612 64-65 -0.22907034982536345 21612 66-67 0.19227661696231024 21612 68-69 0.33210841490461007 21612 70-71 0.013456292805848591 21612 72-73 0.8855067244103054 21612 74-75 0.4771907484814051 21612 76 0.4167144242232297 >>END_MODULE >>Per sequence quality scores pass #Quality Count 10 3.0 11 9.0 12 37.0 13 59.0 14 133.0 15 263.0 16 483.0 17 779.0 18 978.0 19 990.0 20 956.0 21 1040.0 22 1159.0 23 1353.0 24 1529.0 25 1688.0 26 1886.0 27 2221.0 28 2475.0 29 2822.0 30 3379.0 31 4146.0 32 5240.0 33 7712.0 34 12861.0 35 21602.0 36 33137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 65.47824490545254 7.979621810170737 5.077106664218836 21.465026620157886 2 59.24545621442996 8.248577198457866 23.2623462456398 9.243620341472369 3 58.96548558839728 8.895722416008812 23.07600514044428 9.062786855149623 4 58.157317397809784 17.286738449958232 13.765501794582288 10.790442357649694 5 57.778593721314486 13.402790526895538 23.33486322746466 5.4837525243253165 6 63.39269322562878 23.7075454378557 7.212226913897559 5.687534422617955 7 60.77382045162475 12.814393243987515 8.12281990086286 18.288966403524874 8 61.88176978153111 22.97227831834037 7.192032311364054 7.953919588764458 9 59.99173857169083 7.842849274830181 6.676152010280889 25.489260143198088 10-11 62.08004406095098 15.156967137874059 14.284468514778778 8.478520286396181 12-13 61.079952267303106 9.42996144666789 14.815035799522672 14.675050486506333 14-15 67.64044428125574 13.321094180282724 8.716724802643656 10.32173673581788 16-17 66.70598494584175 10.410776574261062 8.008077841013401 14.875160638883788 18-19 61.12623177297283 9.384652808694813 13.999641999843949 15.489473418488414 20-21 67.09060616770013 9.274957889084208 9.438811806661557 14.19562413655411 22-23 67.2457315953736 9.030659078391775 7.734991738571691 15.988617587662935 24-25 62.651459519001286 14.956856985496604 7.997980539746649 14.393702955755462 26-27 67.46557738204515 14.543785570038553 8.517991554984397 9.472645492931889 28-29 67.63952634477694 10.325408481733064 8.814026069395998 13.221039104093995 30-31 61.95153295391959 15.007802460069763 14.068753442261794 8.971911143748851 32-33 67.14262109988158 15.816190712233452 9.11060318159704 7.930585006287923 34-35 62.196163025518636 20.8256838626767 9.077473838810354 7.900679272994308 36-37 67.26087754727374 10.720580135854599 14.50844501560492 7.510097301266752 38-39 67.95133056113973 9.197807947566988 15.283323694911832 7.567537796381461 40-41 67.414631907472 14.378098035615935 10.364879750321277 7.842390306590784 42-43 62.44125206535708 14.740223976500827 14.557095648981091 8.261428309161007 44-45 61.62199375803195 9.125665503947127 15.583348632274646 13.668992105746284 46-47 66.64999059102153 8.361139542035186 10.721186724619855 14.26768314232342 48-49 68.30548926014319 8.284376721130899 15.120708646961631 8.289425371764274 50-51 72.78960895906003 8.501009730126675 10.538369744813659 8.171011565999633 52-53 62.426965673292734 8.735203807652942 15.038301426951902 13.799529092102425 54-55 66.68395447035064 8.681384248210025 9.836607306774372 14.798053974664954 56-57 66.52102074536442 9.064622728107215 9.335872957591334 15.07848356893703 58-59 61.83770883054892 14.478612080044062 14.617220488342205 9.066458601064806 60-61 60.662110621032774 10.058794101313113 15.609122494595622 13.669972783058487 62-63 60.89636497154397 15.076188727740039 14.640627868551496 9.386818432164494 64-65 60.9955021112539 15.300624196805582 15.465393794749405 8.238479897191114 66-67 60.847714338167805 21.680282724435468 8.905819717275564 8.566183220121168 68-69 60.946392509638336 22.124105011933175 8.348632274646594 8.580870203781899 70-71 61.39370935243874 22.02598690098633 8.129741737386349 8.450562009188586 72-73 62.44400587479346 20.986322746465945 8.067284743895723 8.50238663484487 74-75 67.66984587422088 16.1345548344456 7.9582694584943585 8.237329832839164 76 74.89902698733248 9.153662566550395 8.110886726638517 7.836423719478612 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.5 6 0.5 7 1.0 8 2.0 9 2.0 10 2.5 11 6.0 12 9.0 13 5.0 14 4.5 15 8.5 16 11.5 17 14.0 18 19.5 19 24.0 20 34.0 21 45.0 22 67.0 23 100.5 24 112.0 25 112.0 26 148.5 27 230.5 28 307.5 29 339.0 30 385.5 31 472.5 32 622.0 33 731.0 34 807.5 35 929.5 36 1093.0 37 1211.0 38 1331.0 39 1557.5 40 1734.5 41 2056.0 42 2307.0 43 3313.0 44 7253.5 45 6749.5 46 3311.0 47 2976.5 48 2385.0 49 1976.0 50 1824.0 51 1714.5 52 1483.5 53 1300.5 54 1239.0 55 1183.0 56 1017.0 57 839.5 58 772.0 59 729.0 60 665.0 61 625.0 62 606.0 63 512.0 64 413.5 65 380.0 66 321.0 67 291.0 68 280.5 69 266.5 70 232.5 71 202.0 72 212.5 73 191.0 74 166.0 75 173.0 76 167.0 77 167.0 78 172.0 79 171.0 80 170.0 81 160.5 82 158.5 83 165.0 84 169.0 85 159.0 86 179.0 87 213.0 88 209.5 89 215.5 90 232.5 91 289.5 92 339.0 93 369.0 94 448.0 95 554.0 96 611.0 97 802.5 98 1526.5 99 27280.0 100 52501.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 9.179364787956673E-4 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.001376904718193501 20-21 0.001376904718193501 22-23 0.0 24-25 0.0 26-27 0.0 28-29 0.0 30-31 0.0 32-33 9.179364787956673E-4 34-35 0.0 36-37 0.0 38-39 9.179364787956673E-4 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.001376904718193501 48-49 0.0 50-51 0.0 52-53 0.001376904718193501 54-55 0.0 56-57 0.0 58-59 0.0 60-61 4.5896823939783367E-4 62-63 0.0 64-65 0.0 66-67 0.0 68-69 0.0 70-71 4.5896823939783367E-4 72-73 0.0 74-75 0.002753809436387002 76 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 108940.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 25.333210941802825 #Duplication Level Percentage of deduplicated Percentage of total 1 74.31697949126749 18.826877180099135 2 10.28335386622219 5.210207453644208 3 6.471483440829045 4.918303653387186 4 3.938691209507935 3.991187809803562 5 2.076237408507863 2.6298880117495873 6 1.1667512138560765 1.7734532770332292 7 0.7101963910428292 1.2594088489076556 8 0.2971229799260816 0.6021663300899578 9 0.115950431190666 0.26436570589315217 >10 0.5435176462062468 2.3278869102258124 >50 0.04710486267120806 0.858270607673949 >100 0.01811725487354156 0.9867817147053424 >500 0.007246901949416625 1.2327886910225814 >1k 0.0 0.0 >5k 0.0036234509747083126 8.331191481549476 >10k+ 0.0036234509747083126 46.787222324215165 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 50970 46.787222324215165 No Hit CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG 9076 8.331191481549476 No Hit TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT 828 0.7600514044428125 No Hit CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG 515 0.47273728657976866 No Hit ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC 486 0.44611712869469433 No Hit TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG 225 0.20653570772902513 No Hit CTTATACACATCTGACGCTGCCGACGAGCAAGAAAGTGTAGATCTCGGTG 149 0.13677253534055445 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.006425555351569671 0.0 2 0.0 0.0 0.0 0.006425555351569671 0.0 3 0.0 0.0 0.0 0.006425555351569671 0.0 4 0.0 0.0 0.0 0.012851110703139343 0.0 5 0.0 0.0 0.0 0.022030475491096015 0.0 6 0.0 0.0 0.0 0.022030475491096015 0.0 7 0.0 0.0 0.0 0.022030475491096015 0.0 8 0.0 0.0 0.0 0.024784284927483018 0.0 9 0.0 0.0 0.0 0.024784284927483018 0.0 10 0.0 0.0 0.0 0.025702221406278685 0.0 11 0.0 0.0 0.0 0.025702221406278685 0.0 12 0.0 0.0 0.0 0.025702221406278685 0.0 13 0.0 0.0 0.0 0.03212777675784836 0.0 14 0.0 0.0 0.0 0.03304571323664402 0.0 15 0.0 0.0 0.0 0.03304571323664402 0.0 16 0.0 0.0 0.0 0.03304571323664402 0.0 17 0.0 0.0 0.0 0.03304571323664402 0.0 18 0.0 0.0 0.0 0.04130714154580503 0.0 19 0.0 0.0 0.0 0.0449788874609877 0.0 20 0.0 0.0 0.0 0.0449788874609877 0.0 21 0.0 0.0 0.0 0.046814760418579036 0.0 22 0.0 0.0 0.0 0.07068110886726639 0.0 23 0.0 0.0 0.0 0.07068110886726639 0.0 24 0.0 0.0 0.0 0.0872039654855884 0.0 25 0.0 0.0 0.0 0.0927115843583624 0.0 26 0.0 0.0 0.0 0.0927115843583624 0.0 27 0.0 0.0 0.0 0.09730126675234074 0.0 28 0.0 0.0 0.0 0.11107031393427574 0.0 29 0.0 0.0 0.0 0.1349366623829631 0.0 30 0.0 0.0 0.0 0.15972094731044612 0.0 31 0.0 0.0 0.0 0.17532586744997247 0.0 32 0.0 0.0 0.0 0.22397650082614284 0.0 33 0.0 0.0 0.0 0.25059665871121717 0.0 34 0.0 0.0 0.0 0.29924729208738754 0.0 35 0.0 0.0 0.0 0.37818982926381495 0.0 36 0.0 0.0 0.0 0.49935744446484304 0.0 37 0.0 0.0 0.0 0.6563245823389021 0.0 38 0.0 0.0 0.0 0.8656140995043143 0.0 39 0.0 0.0 0.0 1.1345694877914447 0.0 40 0.0 0.0 0.0 1.3640536074903618 0.0 41 0.0 0.0 0.0 1.6275013769047182 0.0 42 0.0 0.0 0.0 1.9102258123737836 0.0 43 0.0 0.0 0.0 2.2590416743161374 0.0 44 0.0 0.0 0.0 2.5867449972461904 0.0 45 0.0 0.0 0.0 2.955755461722049 0.0 46 0.0 0.0 0.0 3.27336148338535 0.0 47 0.0 0.0 0.0 3.6166697264549295 0.0 48 0.0 0.0 0.0 3.882871305305673 0.0 49 0.0 0.0 0.0 4.132550027538095 0.0 50 0.0 0.0 0.0 4.361116210758215 0.0 51 0.0 0.0 0.0 4.652102074536442 0.0 52 0.0 0.0 0.0 4.910960161556821 0.0 53 0.0 0.0 0.0 5.167064439140812 0.0 54 0.0 0.0 0.0 5.464475858270608 0.0 55 0.0 0.0 0.0 5.724251881769781 0.0 56 0.0 0.0 0.0 5.984945841747751 0.0 57 0.0 0.0 0.0 6.223609326234625 0.0 58 0.0 0.0 0.0 6.427391224527263 0.0 59 0.0 0.0 0.0 6.629337249862309 0.0 60 0.0 0.0 0.0 6.856985496603635 0.0 61 0.0 0.0 0.0 7.127776757848357 0.0 62 0.0 0.0 0.0 7.362768496420048 0.0 63 0.0 0.0 0.0 7.581237378373417 0.0 64 0.0 0.0 0.0 7.7740040389205065 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTGTGC 40 1.3278623E-10 70.0 1 CAAACCA 15 0.0022194854 70.0 38 TTGTGCA 20 7.8964826E-5 70.0 2 GGGCTCG 15 0.0022194854 70.0 41 GCGCGTG 20 7.8964826E-5 70.0 6 ATGGACT 15 0.0022194854 70.0 40 CATGAAG 15 0.0022194854 70.0 26 GGCAACC 15 0.0022194854 70.0 20 ACACAGC 15 0.0022194854 70.0 6 TACGCGT 15 0.0022194854 70.0 5 TTCCATA 15 0.0022194854 70.0 16 GTTCCAT 15 0.0022194854 70.0 15 ACGTCTG 30 1.01559635E-7 70.0 8 TAGATTA 15 0.0022194854 70.0 5 GTCCAGC 15 0.0022194854 70.0 10 CACGTCT 30 1.01559635E-7 70.0 7 CGCGTGG 15 0.0022194854 70.0 7 TGTACGC 15 0.0022194854 70.0 3 GATTACT 15 0.0022194854 70.0 7 TGCACGT 15 0.0022194854 70.0 5 >>END_MODULE