FastQCFastQC Report
Wed 25 May 2016
SRR1780075_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780075_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108940
Sequences flagged as poor quality0
Sequence length76
%GC57

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2076219.058197172755644No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1417213.008995777492197No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1115710.241417293923261No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG41003.763539563062236No Hit
ATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCTT9730.8931521938681843TruSeq Adapter, Index 18 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT6670.6122636313567101No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC6410.5883972829080227No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC6180.5672847438957224No Hit
CTTATACACATCTCCGAGCCCACGAGACACGGGCGCATCTCGTATGCCGT4570.41949697080961995No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC4040.37084633743344964No Hit
TATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCG3990.3662566550394713No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT3420.3139342757481182No Hit
ATTATAAACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT2890.2652836423719479No Hit
CTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC2370.21755094547457315No Hit
ATTATACACATCTCCGAGACCACGAGACCCGGGCGCATCTCGTATGCCGT2110.1936845970258858No Hit
ATATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC2050.1881769781531118No Hit
ATATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC1840.16890031209840278No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCAGT1730.15880301083165044No Hit
ATTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCGT1700.15604920139526346No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT1690.15513126491646778No Hit
TATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTCT1690.15513126491646778No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATATCGTATGCCGT1510.13860840829814577No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTAAGCCGT1420.13034697998898476No Hit
CTTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGACGT1340.12300348815861943No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC1280.11749586928584542No Hit
TCTTATACACATCTCCGAGCCCACGAGCCCCGGGCGCATCTCGTATGCCG1270.11657793280704974No Hit
ATTATACACATCTCCGAGCCCACGAGACCAGGGCGCATCTCGTATGCCGT1270.11657793280704974No Hit
ATTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1250.11474205984945841No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGA1240.11382412337066275No Hit
CTATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCC1220.11198825041307142No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGAGCATCTCGTATGCCGT1220.11198825041307142RNA PCR Primer, Index 24 (95% over 21bp)
TTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGTC1210.11107031393427574No Hit
ATTATACACATCTCCGAGCCAACGAGACCCGGGCGCATCTCGTATGCCGT1200.11015237745548008No Hit
TCTTATACACATCTCCGCGCCCACGAGACCCGGGCGCATCTCGTATGCCG1200.11015237745548008No Hit
ATTATACACATCTCCGAGCCCACGAGACCCGGGCGCATCTCGAATGCCGT1180.10831650449788874No Hit
ATTATACACATCTCCGAGCACACGAGACCCGGGCGCATCTCGTATGCCGT1180.10831650449788874No Hit
ATTATACACATCTACGAGCCCACGAGACCCGGGCGCATCTCGTATGCCGT1090.10005507618872775No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTTTGA150.002219485470.019
AGCCGAC207.8964826E-570.045
ACAGTAT150.002219485470.03
ACACGTG150.002219485470.039
CGAGTTG150.002219485470.028
ATCTAAT150.002219485470.032
CAAAAGG150.002219485470.066
GAGTCGA150.002219485470.024
TTGCTGG150.002219485470.032
CCGTCAA207.8964826E-570.047
TGTTGTA150.002219485470.014
GAAGTCT150.002219485470.046
GATTGGG150.002219485470.01
GGGTTAA150.002219485470.024
TCGAGTT150.002219485470.027
AACTCGA150.002219485470.037
TTTGAGG150.002219485470.03
GACGAGG150.002219485470.011
AGTCGAG150.002219485470.025
AGGGGGG42650.065.5685870
Produced by FastQC (version 0.11.5)