FastQCFastQC Report
Wed 25 May 2016
SRR1780074_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780074_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192351
Sequences flagged as poor quality0
Sequence length76
%GC59

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4260822.151171556165554No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG85694.454876761753254No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT7600.3951110209980712No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5610.2916543194472605No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC5160.26825958794079574No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2420.12581166721254375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTGTGTC150.002222081270.0000146
CGCGCGG150.002222081270.0000149
CCTCGCA150.002222081270.0000166
ACGTCAC150.002222081270.0000132
GGCGCGT150.002222081270.000015
TCGACTT150.002222081270.0000119
GTTGGTG150.002222081270.0000143
TGCCGGC252.3860177E-456.00000419
TGGGCGC452.6877387E-854.4444433
TCGCCGT12800.053.86718852
TCTCGGT12950.053.78378343
ATCTCGG12900.053.72093242
CTCGGTG13100.053.7022944
TCGGTGG13100.053.7022945
GCCGTAT12850.053.6575954
TGGTCGC13250.053.62264349
GATCTCG12950.053.5135141
CGCCGTA12900.053.4496153
GTCGCCG13100.053.43511251
AGATCTC13050.053.10344740