FastQCFastQC Report
Wed 25 May 2016
SRR1780074_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780074_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences192351
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT144867.531024013392185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG131166.818784409750924No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGT87694.55885334622643No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG31481.6365914396078003No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT13550.7044413598057717No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT7190.37379582118107No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCGT6620.3441624946062147No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC6330.32908588985760406No Hit
ATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGTCTT5160.26825958794079574RNA PCR Primer, Index 26 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC4410.2292683687633545No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT4350.22614907122915923No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCC4230.21991047616076861No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGACGT3100.1611637059334238No Hit
TCTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTAGTATGCCG2930.15232569625320377No Hit
TATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCG2820.14660698410717907No Hit
CTTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTAAGCCGT2570.133609911048032No Hit
ATTATACACATCTCCGAGCCCACGAGACGTTCGAGTATCTCGTATGCCGA2470.12841108182437314No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGTA150.002222964869.9929845
TGACGAC150.002222964869.9929845
CCGTCAA150.002222964869.9929847
GCCGACA150.002222964869.9929846
TAAGACG207.915802E-569.9929843
AGGGGGG30650.063.71302870
ATTATAC25800.061.4633031
ATTCCGT352.9649163E-759.99398844
GCCGACT700.059.99398846
GTATGAC2550.059.0136942
CGTAATC950.058.94145648
GACGTAT308.358667E-658.32748446
TAACTCG308.358667E-658.32748436
TGCCGAC850.057.64128545
AGCCGTC1350.057.03131545
ACGATCG800.056.88408727
GTATTCT1250.055.9943949
TTCCGTC252.3872005E-455.99438545
CGAATTA252.3872005E-455.99438548
AACTCGT252.3872005E-455.99438537
Produced by FastQC (version 0.11.5)