FastQCFastQC Report
Wed 25 May 2016
SRR1780073_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780073_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109280
Sequences flagged as poor quality0
Sequence length76
%GC68

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3996036.566617862371885No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG79647.2877013177159595No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT7410.6780746705710102No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4340.39714494875549045No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG4130.3779282576866764No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2520.23060029282576866No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1220.11163982430453881No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACGAT252.82381E-670.022
TACGCGT150.002219504770.05
CGGATAG150.002219504770.053
CGCGTGT150.002219504770.07
CGCGTGG150.002219504770.07
TGTGGCG352.9509283E-760.011
GCGCGTG308.3296545E-658.3333326
GGCGCGT308.3296545E-658.3333325
CTATACA252.3814393E-455.9999961
CCAACCG252.3814393E-455.9999968
CTTTATA452.6733687E-854.4444472
TGCCGGC452.6733687E-854.44444719
GACAGGG200.006935294752.521
TTGTGCG407.421604E-752.52
GGTCGCA200.006935294752.552
AGCCCCC200.006935294752.541
GGACTGG200.006935294752.51
TGGCGCG200.006935294752.513
GTCTCGG200.006935294752.542
AGGAACT200.006935294752.542