FastQCFastQC Report
Wed 25 May 2016
SRR1780073_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780073_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences109280
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT1515613.86896046852123No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1182710.822657393850658No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT1150910.531661786237189No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG40673.7216325036603224No Hit
ATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCTT8340.763177159590044RNA PCR Primer, Index 39 (95% over 22bp)
CTCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC6620.6057833089311859No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC6190.5664348462664714No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC5260.4813323572474377No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT3210.29374084919472915No Hit
TATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCG3110.2845900439238653No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTAGTATGCCGT2470.22602489019033672No Hit
ATTATAAACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGT2180.1994875549048316No Hit
CTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC2030.18576134699853586No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTAAGCCGT1830.1674597364568082No Hit
TATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTCT1600.14641288433382138RNA PCR Primer, Index 39 (95% over 21bp)
ATTATACACATCTCCGAGACCACGAGACGCATGTACATCTCGTATGCCGT1490.13634699853587115No Hit
ATATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC1480.13543191800878476No Hit
ATATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGTC1440.13177159590043924No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCAGT1350.12353587115666179No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATATCGTATGCCGT1260.11530014641288433No Hit
TCTTAATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC1250.11438506588579796No Hit
CTTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGACGT1250.11438506588579796No Hit
ATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCCGA1230.11255490483162518No Hit
CTATTATACACATCTCCGAGCCCACGAGACGCATGTACATCTCGTATGCC1140.10431918008784773No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAATA207.8965975E-570.02
CTAGACG150.002219504770.023
AGCCGAC252.82381E-670.045
ATCTCGA500.070.037
GTACAAC301.0156327E-770.033
TAGACGC150.002219504770.024
CGTCTAC252.82381E-670.048
ATATCGA207.8965975E-570.037
TAATTGC150.002219504770.07
CCATATC150.002219504770.01
ACTCGTA150.002219504770.038
GGACGAA150.002219504770.042
TTCGGGG150.002219504770.050
CGACTTA150.002219504770.048
GACGAAC150.002219504770.043
CGTCCTC150.002219504770.05
TCGAATG850.070.040
ACGAACC150.002219504770.044
GCCGTAT900.066.11111546
ATTATAC21050.066.009511
Produced by FastQC (version 0.11.5)