FastQCFastQC Report
Wed 25 May 2016
SRR1780072_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780072_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187428
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5130827.37477858164202No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG70063.737968713319248No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT6420.3425315321083296No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5560.29664724587574964No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4150.22141835798279874No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2310.12324732697355785No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTGT252.831719E-670.000017
ACACGTC150.002221992270.06
ACACGTA150.002221992270.025
GACACGT150.002221992270.024
GGTCACG150.002221992270.028
ATCGTAC150.002221992270.012
GTCACGG150.002221992270.029
GTTCACG150.002221992270.03
CATCGTA150.002221992270.011
ACGTACT150.002221992270.027
CACGTGA150.002221992270.06
CACGTCT150.002221992270.07
CACGTAC150.002221992270.026
TACACGT150.002221992270.05
TCACGTG150.002221992270.05
GCGTGTG308.35287E-658.3333328
TCGTTGT200.00694300652.534
CCAGGAA200.00694300652.53
GTTTAGC200.00694300652.51
CGTGACG200.00694300652.58
Produced by FastQC (version 0.11.5)