FastQCFastQC Report
Wed 25 May 2016
SRR1780072_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780072_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences187428
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT2460313.126640630001921No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG108465.786755447425144No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT87824.685532577843225No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG36021.9218046396482915No Hit
ATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTCTT11680.6231726316238768Illumina PCR Primer Index 3 (95% over 21bp)
ATCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC8230.4391019484815502No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC6770.36120536952856563No Hit
TATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCG5750.30678447190387775No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGACGT4700.25076295964316964No Hit
ATTATAAACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGT4390.23422327507096058No Hit
ATATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC4040.21554943765072454No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCC2890.154192543269949No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGA2760.14725654651386133No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATATCGTATGCCGT2410.1285827090936253No Hit
ATTATACACATCTCCGAGACCACGAGACTCGAAGCCATCTCGTATGCCGT2360.12591501803359156No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCAGT2330.12431440339757134No Hit
CTATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC2180.11631133021747019No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGCAATCTCGTATGCCGT2150.11471071558144993No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGAAGACATCTCGTATGCCGT2110.11257656273342297RNA PCR Primer, Index 41 (95% over 21bp)
ATATACACATCTCCGAGCCCACGAGACTCGAAGCCATCTCGTATGCCGTC1970.10510702776532854No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGAATC150.002217995570.0317729
AATCCAT150.002220345470.0130832
CAACTTC150.002220345470.0130825
AACGTAT150.002222697369.9943939
CGTGACC150.002222697369.9943933
TGACGAC207.914477E-569.9943945
GTCACGT150.002222697369.9943954
TCGTCAC150.002222697369.9943952
CACGTGA150.002222697369.9943956
CGTCACG150.002222697369.9943953
ATATCGT800.065.6197437
ATTATAC35450.064.268481
AGGGGGG40150.064.1542770
CGTCTTA950.062.62656448
CAACTCG950.062.62656436
ATCGTAT900.062.21724339
GCCAACT900.062.21724334
GCGAACT401.0568328E-861.27779828
AGCCGTC750.060.6618145
TCGAATG750.060.6618140
Produced by FastQC (version 0.11.5)