FastQCFastQC Report
Wed 25 May 2016
SRR1780071_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780071_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161029
Sequences flagged as poor quality0
Sequence length76
%GC70

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG6198838.4949294847512No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG101356.293897372522962No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT8800.5464854156704693No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG6610.410485067907023No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC5960.3701196678859087No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2980.18505983394295436No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1960.12171720621751363No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCGC308.350617E-658.3297123
CGACGAC252.385577E-455.9965222
TACACGT252.385577E-455.996525
ATACACG252.385577E-455.996524
AAAAAGG11900.055.5847870
CCGTATC14300.054.3322955
TCGGTGG14950.054.3110145
GCCGTAT14450.054.2526854
CTCGGTG14800.054.15204244
ATCTCGG14750.054.0983342
GGTGGTC14900.054.04026847
TCGCCGT14450.054.02726452
CGGTGGT14900.054.0234946
CGTATCA14450.054.01048356
TCTCGGT14850.053.9697143
TAGATCT14800.053.9155739
AGATCTC14900.053.78860540
GTCGCCG14650.053.76762851
TGGTCGC14850.053.75072549
CGCCGTA14400.053.72862653