FastQCFastQC Report
Wed 25 May 2016
SRR1780071_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780071_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences161029
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT2805217.42046463680455No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG155189.63677350042539No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT103746.442317843369828No Hit
TCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG43432.6970297275646002No Hit
ATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCTT13360.8296642219724398TruSeq Adapter, Index 3 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC9130.5669786187581118No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC8250.5123300771910649No Hit
TATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCG6600.40986406175285195No Hit
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT5770.35832055095666No Hit
ATTATAAACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT5360.33285929863564945No Hit
ATTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT3840.23846636320165931No Hit
ATATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC3680.22853026473492352No Hit
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATATCGTATGCCGT3350.20803706164728092No Hit
ATATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC3170.19685895087220312TruSeq Adapter, Index 3 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGA2930.18195480317209944No Hit
CTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC2820.17512373547621857TruSeq Adapter, Index 3 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCAGT2700.1676716616261667No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC2640.1639456247011408No Hit
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTAAGCCGT2500.15525153854274695No Hit
TTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTC2390.1484204708468661TruSeq Adapter, Index 3 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTAGTATGCCGT2370.14717845853852413No Hit
CTTATACACATCTCCGAGCCCACGAGACAACTAGGCATCTCGTATGCCGT2340.14531544007601116No Hit
ATTATACACATCTCCGAGACCACGAGACCACTAGGCATCTCGTATGCCGT2310.1434524216134982No Hit
ATTATACACATCTCCGAGCCAACGAGACCACTAGGCATCTCGTATGCCGT2240.13910537853430127No Hit
TATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGTCT2190.13600034776344633RNA PCR Primer, Index 3 (95% over 22bp)
ATTATACACATCTCCGAGCCCACGAGACCACTAGGCATCACGTATGCCGT1920.11923318160082966No Hit
TCTTAATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGCC1820.1130231200591198No Hit
CTTATACACATCTCCGAGCCCACGAGACCACTAGGCATCTCGTATGACGT1740.10805507082575189No Hit
ATTATACACATATCCGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT1720.10681305851740991No Hit
ATTATACACATCTCAGAGCCCACGAGACCACTAGGCATCTCGTATGCCGT1690.10495004005489696No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGACC150.002219857270.0124319
CGACTAG150.002219857270.0124328
AAGCTTG500.070.0124354
GACACGT150.002222594669.99068522
GACCGAA150.002222594669.99068522
TAACGTA207.913173E-569.99068538
TGACGAC207.913173E-569.99068545
TTCCCGG150.002222594669.99068543
CTAAAAG150.002222594669.9906853
ACTAAGG150.002222594669.99068548
CATAACG150.002222594669.99068536
ATAACGT150.002222594669.99068537
TAAGACG252.8321429E-669.99068543
GCCGTAT750.065.3246446
ATTATAC38550.065.0886151
TCTTTAT1400.064.991351
AGGGGGG46100.063.9936570
AGCCGTC1100.063.62789545
CCGTATT800.061.2418547
ATGACGA401.0604708E-861.2418544
Produced by FastQC (version 0.11.5)