FastQCFastQC Report
Wed 25 May 2016
SRR1780070_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780070_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79532
Sequences flagged as poor quality0
Sequence length76
%GC87

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG5479668.8980536136398No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG896811.275964391691394No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT8721.096414022028869No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC5690.7154352964844339No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG5030.632449831514359No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG3210.40361112508172814No Hit
CTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGG1400.17602977417894686No Hit
CTTATACACATCTGACGCTGCCGACGAGCAAGAAAGTGTAGATCTCGGTG1130.1420811748730071No Hit
CTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT1070.13453704169390937No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1040.1307649751043605No Hit
GGGGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG940.11819141980586431No Hit
CTTTACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGG880.1106472866267666No Hit
GGGGGGCGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG800.10058844238796961No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGAC207.885837E-569.995616
ACGCTGA207.885837E-569.995615
CTATACA301.0125041E-769.99561
GATATCG150.00221782769.995641
GTCGCGG150.00221782769.995651
GTGTAGG150.00221782769.995636
TATCGGT150.00221782769.995643
TCGCGGT150.00221782769.995652
GCGGTAT150.00221782769.995654
CGTGGGC252.8177783E-669.99561
ATCGGTG150.00221782769.995644
GCGCTGC301.0125041E-769.995615
GCTGACG207.885837E-569.995617
GACGACG207.885837E-569.995620
CTTGTGC150.00221782769.99561
CTGCCGG301.0125041E-769.995618
ACGACGA207.885837E-569.995621
TGGCGCG207.885837E-569.995613
ATATCGG150.00221782769.995642
GGTATCA150.00221782769.995656
Produced by FastQC (version 0.11.5)