FastQCFastQC Report
Wed 25 May 2016
SRR1780070_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780070_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79532
Sequences flagged as poor quality0
Sequence length76
%GC58

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT2484731.241512850173514No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1371717.24714580294724No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT862210.840919378363425No Hit
TCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG37744.745259769652467No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCTT11611.459789770155409TruSeq Adapter, Index 11 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC9661.2146054418347332No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC6340.7971634059246592No Hit
TATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG4690.589699743499472No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT4580.5758688326711261Illumina PCR Primer Index 6 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGACGT4260.5356334557159382No Hit
ATTATAAACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT4080.5130010561786451No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTAAGCCGT3550.44636121309661514No Hit
CTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC3440.43253030226826933No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCACACATCTCGTATGCCGT3350.4212141024996228No Hit
ATATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC3230.40612583614142733No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTAGTATGCCGT3020.37972137001458534No Hit
ATATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC2900.36463310365638985No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC2510.3155962379922547No Hit
TATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCT2370.29799326057436No Hit
ATTATACACATCTCCGAGACCACGAGACTCGCCCACATCTCGTATGCCGT2290.28793441633556305No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC2250.28290499421616455No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGA2220.2791329276266157No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCAACATCTCGTATGCCGT2180.27410350550721724Illumina PCR Primer Index 6 (95% over 21bp)
ATTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG2010.2527284614997737No Hit
TTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC1990.25021375044007443No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCAGT1860.23386812855202938No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCACGTATGCCGT1830.2300960619624805No Hit
ATTATACACATCTCCGAGCCAACGAGACTCGCCCACATCTCGTATGCCGT1820.22883870643263088No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGACCACATCTCGTATGCCGT1640.2062063068953377RNA PCR Primer, Index 41 (95% over 21bp)
ATTTACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC1580.19866217371624No Hit
ATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTCAT1370.17225770758939798No Hit
ATTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG1340.1684856409998491No Hit
CTTTACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGTC1300.16345621888045064No Hit
CTATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC1240.15591208570135293No Hit
ATTATACACATCTCAGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1220.15339737464165368No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATATCGTATGCCGT1220.15339737464165368No Hit
TCTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT1150.14459588593270634No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTAAGCCGT1150.14459588593270634No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACAACTCGTATGCCGT1140.1433385304028567No Hit
ATTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGAATGCCGT1120.1408238193431575No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGACGT1120.1408238193431575No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCACACATCTCGTATGCCGT1100.13830910828345822No Hit
ATTATACACATCTCCGAGCACACGAGACTCGCCCACATCTCGTATGCCGT1040.1307649751043605No Hit
CTTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG1010.12699290851481165No Hit
ATTATACACATATCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT960.12070613086556355No Hit
ATTATACACATCTACGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT950.11944877533571392No Hit
ACTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG940.11819141980586431No Hit
CTTAATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCG920.11567670874616508No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGACCACATCTCGTATGCCGT920.11567670874616508No Hit
CTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGA900.11316199768646583No Hit
ATTATACACATCACCGAGCCCACGAGACTCGCCCACATCTCGTATGCCGT820.10310315344766885No Hit
TATTTATACACATCTCCGAGCCCACGAGACTCGCCCACATCTCGTATGCC800.10058844238796961No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACAACA252.8081522E-670.0358731
ACAACAT252.8081522E-670.0358732
CTCGACA207.863375E-570.03586628
TCGACAA207.863375E-570.03586629
CCACAAC550.069.9918333
CTACAGC207.887944E-569.9918251
ATGGGGG150.002218301269.9918270
AGGGGGC150.002218301269.9918270
AGGGGCG150.002218301269.9918270
ACTGCAT150.002218301269.9918253
TCAAGTA150.002218301269.9918238
ATCTCGA252.8186805E-669.9918237
ATCTTAT700.069.991821
TCTAGAA150.002218301269.9918238
TATACAA401.3278623E-1069.991823
ACGTCTC150.002218301269.9918235
ACTTGAA207.887944E-569.9918256
CCACGTC207.887944E-569.9918233
ATGACGA150.002218301269.9918244
ACGTCAT150.002218301269.9918247
Produced by FastQC (version 0.11.5)