FastQCFastQC Report
Wed 25 May 2016
SRR1780069_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1780069_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences146628
Sequences flagged as poor quality0
Sequence length76
%GC61

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3789825.846359494775896No Hit
CTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTG69234.721472024442807No Hit
TCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGT5750.39214883923943583No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG4770.32531303707341025No Hit
ATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGGTGGTC4690.31985705322312247No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCTAGAAAGTGTAGATCTCGG2100.14321957607005484No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCGT150.002221024170.052
CCGGCGA150.002221024170.021
ATCGTAC150.002221024170.03
TCGTACT150.002221024170.04
CATCGTA150.002221024170.02
ACATCGT150.002221024170.01
TGCCGGC352.9579678E-760.019
CTCAGTC252.3841386E-455.99999640
GCGACAA200.006940005352.541
GGGCTAA200.006940005352.51
GCGAATA200.006940005352.539
GACACGT200.006940005352.515
TAGGTAC200.006940005352.53
TCGGGCT200.006940005352.557
CGACACG200.006940005352.514
TGTGCGC200.006940005352.53
GGTGGAC200.006940005352.547
TCTGTAG200.006940005352.537
CAGTGTA200.006940005352.59
GTTCCAA200.006940005352.51
Produced by FastQC (version 0.11.5)